Anti-Doublecortin Antibody

Name: Anti-Doublecortin Antibody
Brand: Aves Labs
SKU: DCX
Price: 481.0 USD
Availability: InStock

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (AAT58219.1), rat (NP_445831.3) and human (CAA06617.1) gene products. These antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Doublecortin (1:1000 dilution, green) staining of neuroblastoma cells in culture. Red staining is a Golgi apparatus marker. Page Balisch (University of Arizona).

Doublecortin (1:1000 dilution, green) staining of a tissue section (4% paraformaldehyde-fixed, paraffin- embedded) through the cochlear ganglion from a neonatal mouse. Red staining is neurofilament, NF-M, visualized with Texas Red-goat anti-rabbit IgG.

Doublecortin (1:1000 dilution, green) staining of a tissue section (4% paraformaldehyde-fixed, paraffin- embedded) through the cerebellar cortex of a neonatal mouse. Red staining is neurofilament, NF- M, visualized with Texas Red-goat anti-rabbit IgG.

Doublecortin (1:1000 dilution, red) staining of a tissue section (4% paraformaldehyde-fixed, paraffin-embedded) through the cerebral cortical periventricular zone cerebellar cortex of a neonatal mouse. Green staining is neurofilament, NF-M, visualized with fluorescein-goat anti-rabbit IgG.

Doublecortin (1:1000 dilution, green) staining of neuroblastoma cells in culture. Red staining is a Golgi apparatus marker. Page Balisch (University of Arizona).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

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Data Sheet

Trial Size

SKU: DCX

Volume: 1000 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Doublecortin

Scientific Background

Doublecortin is a microtubule-associated protein found in neuroblasts and immature neurons, especially those undergoing embryonic migrations. It was first identified as the X-chromosome-encoded gene product mutated in the human diseases Lissencephaly (in males) and Periventricular Heterotopia (in females), diseases caused by failure of neocortical neuroblast migration. The human protein itself is 360 amino acids (40,044 daltons) and contains doublecortin motifs near its N-terminus that are believed to mediate the microtubule binding.

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

DCX DBCN LISX

Molecular Weight

40 kDa

Immunogen

Synthetic peptide

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313540

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the doublecortin gene product, but are shared between the human (CAA06617.1, NCBI) and mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of these affinity-purified antipeptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Neuronal migration protein doublecortin (Doublin) (Lissencephalin-X) (Lis-X)

UniProt ID

O15075

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Anti-Doublecortin Antibody

SKU: DCX

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