Our Anti-DARPP-32 (Thr75) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat DARPP-32 (Thr75) and is antigen affinity purified. It is great for use in WB.
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr75 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Antigen Affinity Purified
Polyclonal
IgG
ELISA, WB
Rabbit
PPP1R1B
32 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat DARPP-32 conjugated to keyhole limpet hemocyanin (KLH).
Rat
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate
AB_2492069
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr75. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Thr75
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
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