Anti-CSPG4/NG2 Antibody (6203-34)

Name: Anti-CSPG4/NG2 Antibody
Brand: Antibodies Incorporated
SKU: 77-523
Price: 337.0 USD
Availability: InStock

Our mouse CSPG4/NG2 monoclonal antibody from Antibodies Incorporated is produced in-house from clone 6203-34. It detects human, mouse, and rat CSPG4/NG2 and is purified by Protein A chromatography. Works in WB, IHC, ELISA.

Western blotting of mouse or rat brain homogenate (10 ug/lane) with Antibodies Incorporated anti-CSPG4/NG2 antibody at 1:50 dilution and detected with anti-mouse HRP.

Sagittal section of formalin-fixed, paraffin-embedded rat brain showing strong staining for CSPG4/NG2 in the cerebellum, hippocampus and cortex (A-C respectively). Sections were stained with Antibodies Incorporated mouse anti-CSPG4/NG2 (clone 6203-34) antibody at 1:40 dilution and detected with anti-mouse HRP.

Western blotting of mouse or rat brain homogenate (10 ug/lane) with Antibodies Incorporated anti-CSPG4/NG2 antibody at 1:50 dilution and detected with anti-mouse HRP.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 77-523

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CSPG4/NG2

Scientific Background

Neuron-glial antigen 2 (NG2) is a chondroitin sulfate proteoglycan that is encoded by the CSPG4 gene in humans. It is an excellent oligodendrocyte marker. NG2 is a hallmark protein of oligodendrocyte progenitor cells (OPCs) and has been linked with familial schizophrenia (de Vrij, F.M., et al, 2018). NG2 also plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells (Luo, W., et al, 2006). NG2 localizes at the apical plasma membrane and relocalizes to the lamellipodia of astrocytoma upon PRKCA phosphorylation.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

6203-34

Isotype

IgG1

Applications

ELISA, IHC, WB

Host Species

Mouse

Gene Name

CSPG4/NG2

Molecular Weight

250 kDa

Immunogen

Fusion protein amino acids S1583-S2224 of human CSPG4/NG2 (accession number Q6UVK1) produced in CHO cells

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2938812

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:50
WB Brain: 1:50
IHC: 1:40

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

UniProt ID

Q6UVK1

Shipping

Shipped on ice packs

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Anti-CSPG4/NG2 Antibody (6203-34)

SKU: 77-523

Product Details

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