Anti-Complement C9 Antibody (22)
Our mouse Complement C9 monoclonal antibody from Antibodies Incorporated is produced in-house from clone 22. It detects human Complement C9 and is Protein A or Protein G purified. Works in ELISA, IF, IHC, WB.
Immunofluorescent staining of Complement C9 in Follicular dendritic cell network in germinal centers of human tonsil tissue using Anti-Complement C9 Antibody (Cat. ABS 004-22-010). Cells where fixed with formaldehyde and permeabilized prior to staining Primary antibody (ABS 004-22-010) was incubated with the cells at a dilution of 1:75.
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SKU: ABS 004-22-010
Ships: 1-2 business days
Product Details
Complement C9
Complement factor C9 is a 71 kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 µg/mL, and the level is up regulated from hepatocytes by IFN-ɣ.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
22
IgG1/k
ELISA, IF, IHC, WB
Mouse
C9
71 kDa
Complement factor C9
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
IHC: 1:100
ELISA: 1:50
ELISA: 1:50
ABS 004-22 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate. ABS 004-22 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100. ABS 004-22 was used in IF with a dilution of 1:75.
Unconjugated
ABS 004-22 is specific for complement factor C9.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
C9, ARMD15, C9D, complement component C9, complement component 9
735
Shipped on ice packs

