Anti-Clcn3 Antibody (N258/5)

Name: Anti-Clcn3 Antibody
Brand: NeuroMab
SKU: 75-259
Price: 337.0 USD
Availability: InStock

Our mouse Clcn3 monoclonal antibody from NeuroMab is produced in-house from clone N258/5. It detects human, mouse, and rat Clcn3 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.

Adult rat brain immunohistochemistry.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-259

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Clcn3

Scientific Background

Chloride Voltage-Gated Channel 3, CLCN3 or H(+)/Cl(-) exchange transporter 3 is encoded by the gene CLCN3 and is a member of the voltage-gated chloride channel (ClC) family. CLCN3 is a proton-coupled chloride transporter. It plays a role in acidification of endosomes and synaptic vesicle lumen. CLCN3 implicated in vesicle trafficking, neuronal cell function and short term memory. CLCN3 is expressed in several tissues including brain, aortic and coronary vascular smooth muscle cells, bronchus and colon. Diseases associated with this gene include Dent Disease 1 and Cystic Fibrosis

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N258/5

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

CLCN3

Molecular Weight

90 kDa

Immunogen

Synthetic peptide amino acids 98-115 (CKDRERHRRINSKKKESA, cytoplasmic N-terminus) of human Clcn3 (accession number P51792)

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10671699

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

H(+)/Cl(-) exchange transporter 3 (Chloride channel protein 3) (ClC-3) (Chloride transporter ClC-3)

UniProt ID

P51790

Shipping

Shipped on ice packs

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Anti-Clcn3 Antibody (N258/5)

SKU: 75-259

Product Details

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