Our Anti-Calnexin chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Calnexin, and is affinity-purified IgY. Works in ICC, IHC, WB.
Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Calnexin (CANX) (Cat. CANX-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-Calnexin specifically stains the Endoplasmic Reticulum in HeLa cells.
Calnexin is a vital chaperone protein involved in the quality control of glycoprotein folding within the endoplasmic reticulum (ER), facilitating correct folding and preventing aggregation. Calnexin’s calcium-dependent binding highlights its role in calcium homeostasis within the ER. The importance of calnexin extends beyond protein folding; it is crucial for immune function, as it assists in the assembly of major histocompatibility complex (MHC) class I molecules, thus playing a part in antigen presentation. Defects or dysregulation in calnexin prominently feature in cystic fibrosis (Pind et al. 1994), where misfolding of the CFTR glycoprotein is not efficiently managed by the calnexin cycle, leading to its degradation and resulting in disease. Due to its importance in protein folding and homeostasis, calnexin dysfunction can also lead to neurodegenerative disorders including Alzheimer’s, Parkinson’s, ALS, and Huntington’s. Dysregulation of these processes can also contribute to tumor development and progression, making calnexin a critical player in cancers including breast, prostate, liver, and lung. Recent studies have identified calnexin as a dual-role biomarker in lung cancer by utilizing anti-Calnexin antibodies for both diagnostic and therapeutic targeting (Lim et al. 2024).
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
CANX
90 kDa
Synthetic peptide
Human, Mouse, Rat
Human, Mouse, Rat
AB_3105808
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe HeLa cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Calnexin, IP90, P90, CNX, Major Histocompatibility Complex Class I Antigen-Binding Protein P88, Epididymis Secretory Sperm Binding Protein
Ice packs
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