Anti-Beta4-Spectrin Antibody (N393/76)

Name: Anti-Beta4-Spectrin Antibody
Brand: NeuroMab
SKU: 75-377
Price: 394.0 USD
Availability: InStock

Our mouse Beta4-spectrin monoclonal antibody from NeuroMab is produced in-house from clone N393/76. It detects human, mouse, and rat Beta4-spectrin and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat cerebral cortex immunohistochemistry.

Hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment showing axon initial segment and nodal staining.

Higher magnification of corpus callosum and CA1.

Immunoblot against extracts of COS cells transiently transfected with tagged Beta-spectrins or untagged Kv2.1 plasmid probed with N393/76 (left) or 19E10 (right) TC supe.

Immunocytochemistry for α1, α2, and β4 spectrin (Cat. 75-377, 1:500) carried out in fixed and permeabilized DIV 21 primary cultured pHα2 mouse neurons. Image from publication CC-BY-4.0. PMID:36604600

Confocal images of primary hippocampal neurons stained with phalloidin-647 (F-actin marker) and with an antibody against beta4-spectrin (Antibodies Inc; Cat. 75-377, 1:500) to identify the axon initial segment. Image from publication CC-BY-4.0. PMID: 38525600

Adult rat cerebral cortex immunohistochemistry.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-377

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Beta4-spectrin

Scientific Background

Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N393/76

Isotype

IgG2b

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

SPTBN4 KIAA1642 SPTBN3

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315818

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:250
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with other Beta-spectrins

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

UniProt ID

Q9H254

Shipping

Shipped on ice packs

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Anti-Beta4-Spectrin Antibody (N393/76)

SKU: 75-377

Product Details

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