Anti-Beta1-Spectrin Antibody (N385/21)
Our mouse Beta1-spectrin monoclonal antibody from NeuroMab is produced in-house from clone N385/21. It detects human, mouse, and rat Beta1-spectrin and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.
Immunofluorescence staining of optic nerve from adult Beta4-spectrin wild-type (WT) and conditional knockout (cKO) mice with N385/21 (Beta1- spectrin, red), Beta4- spectrin antibody (green) and Neurofascin antibody (blue). Images courtesy of Cheng-Hsin Liu and Matt Rasband (Baylor College of Medicine).
Click on image to zoom
Human, Mouse, Rat
ELISA, ICC, IHC, IP, WB
Mouse
SKU: 75-374
Ships: 1-2 business days
Product Details
Beta1-spectrin
Spectrin beta chain, erythrocytic, Beta1 spectrin, or Membrane Cytoskeletal Protein is encoded by the gene SPTB and is a member of the member of the spectrin gene family. Beta1 spectrin is primary member of the cytoskeletal network in the erythrocyte plasma membrane. Beta1 spectrin is involved in cell membrane organization and stability and associates with the proteins band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Diseases associated with this gene include Spherocytosis and Elliptocytosis.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N385/21
IgG2b
ELISA, ICC, IHC, IP, WB
Mouse
SPTB SPTB1
250 kDa
Fusion protein amino acids 1555-2137 (C-terminal repeats 13 to 17) of human Beta1-spectrin (accession number P11277) produced recombinantly in E. Coli
Human
Human, Mouse, Rat
AB_2315815
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:200
ICC: 1:200
IHC: 1:200
ICC: 1:200
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Spectrin beta chain, erythrocytic (Beta-I spectrin)
Shipped on ice packs

