Anti-Beta-Tubulin 3 (TUJ) Antibody
Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.
Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr. Gerry Shaw (EnCor Biotechnology).
Click on image to zoom
SKU: TUJ
Ships: 1-2 business days
Product Details
Beta-Tubulin 3
Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.
Affinity-Purified IgY
300 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
TUBB3 TUBB4
50 kDa
Synthetic peptides
Human
Human, Mouse, Rat
AB_2313564
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:10000-1:20000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Tubulin beta-3 chain (Tubulin beta-4 chain) (Tubulin beta-III)
Ice packs

