Anti-Bassoon Recombinant Chicken Chimeric mAb (BSA and Azide Free) (L124/59)

Name: Anti-Bassoon Recombinant Chicken Chimeric mAb (BSA and Azide Free)
Brand: Aves Labs
SKU: 78-491-CF
Price: 522.0 USD
Availability: InStock

Our chicken Anti-Bassoon carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L124/59. It detects mouse and rat Bassoon and is purified by affinity chromatography. Works in WB, IHC, ICC, ELISA, AT.

Anti-Bassoon Recombinant Chicken Chimeric mAb (BSA and Azide Free)

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Applications

AT, ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

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Data Sheet

SKU: 78-491-CF

Volume: 100 µL

Variant

Price:

$522.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Bassoon

Scientific Background

Bassoon is a large (420-kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999).

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.6-1.0 mg/mL

Clonality

Recombinant

Clone

L124/59

Isotype

IgY

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Bsn

Molecular Weight

400 kDa for full-length protein, many smaller bands possible depending on sample used

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli

Immunogen Species

Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Buffer

PBS

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated, Carrier Free

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Protein bassoon

UniProt ID

O88778

Shipping

Shipped on ice packs

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Anti-Bassoon Recombinant Chicken Chimeric mAb (BSA and Azide Free) (L124/59)

SKU: 78-491-CF

Product Details

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