Anti-Bassoon Recombinant Chicken Chimeric mAb (L124/59)

Name: Anti-Bassoon Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-491
Price: 481.0 USD
Availability: InStock

Our chicken Anti-Bassoon recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L124/59. It detects mouse and rat Bassoon and is purified by affinity chromatography. Works in WB, IHC, ICC, ELISA, AT.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with anti-Bassoon recombinant chicken monoclonal antibody (green) (Cat. 78-491) at 1:100 dilution. The sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Bassoon stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 100X.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with anti-Bassoon recombinant chicken monoclonal antibody (green) (Cat. 78-491) at 1:100 dilution. The sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Bassoon stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 100X.

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Species Reactivity

Mouse, Rat

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-491

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Bassoon

Scientific Background

Bassoon is a large (420 kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999).

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

L124/59

Isotype

IgY

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Bsn

Molecular Weight

400 kDa for full-length protein, many smaller bands possible depending on sample used

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Protein bassoon

UniProt ID

O88778

Shipping

Shipped on ice packs

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Anti-Bassoon Recombinant Chicken Chimeric mAb (L124/59)

SKU: 78-491

Product Details

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