Anti-Atrogin-1 Antibody (M314)

Name: Anti-Atrogin-1 Antibody
Brand: PhosphoSolutions
SKU: AM3141
Price: 348.0 USD
Availability: InStock

Our Atrogin-1 rat rat monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Atrogin-1 and is antigen affinity purified. It is great for use in WB.

Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rat

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Data Sheet

SKU: AM3141

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Atrogin-1

Scientific Background

Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M314

Applications

ELISA, WB

Host Species

Rat

Gene Name

Fbxo32

Molecular Weight

Immunogen

Clone M314 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

This antibody detects a full length human recombinant Atrogin 1 on SDS-PAGE immunoblots.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

MAFbx, FBX32, Atrogin

UniProt ID

Q9CPU7

Shipping

Blue Ice

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Anti-Atrogin-1 Antibody (M314)

SKU: AM3141

Product Details

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