Anti-Ankyrin-G (Staining) Antibody (N106/36)

Our mouse Ankyrin-G (Staining) monoclonal antibody from NeuroMab is produced in-house from clone N106/36. It detects feline, hamster, human, mouse, non-human primate, and rat Ankyrin-G (Staining) and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Immunofluorescence staining of nodes of Ranvier in adult rat optic nerve with N106/36 (red) and Caspr rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Adult rat cortex immunohistochemistry of axon initial segments.
Adult rat thalamus immunohistochemistry of axon initial segments.
Immunostaining of mouse WT neurons (A) or neurons transfected with BFP vector (B), or BFP-Nfasc-shRNA (C) and stained for AnkG (red), Nfasc (green), and MAP2 (white). Image from publication CC-BY-4.0. PMID: 35850303
Immunostaining of V5-labeled Nav1.2 and Nav1.6 with MAP2 or AnkG in cultured rat hippocampal neurons. Image from publication CC-BY-4.0. PMID: 35672149
Immunostaining of of Nav1.2 and Nav1.6 labeled with V5 tag in mouse CA1 costained with AnkG(red). Arrowheads indicate Nav1.2 or Nav1.6-positive region with AnkG signals. DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35672149
Cultured rat hippocampal neurons (DIV 11–12) were treated for 48 h with 1 μM TTX or its vehicle control (0.1% H2O) and subjected to immunocytochemistry with antibodies for the AIS marker ankyrin-G or somatodendritic marker MAP2. Image from publication CC-BY-4.0. PMID: 35966206
Cultured rat hippocampal neurons (DIV 11–12) were treated for 48 h with 1 μM TTX or its vehicle control (0.1% H2O) and subjected to immunocytochemistry with antibodies for the AIS marker ankyrin-G or Kv7.3. Image from publication CC-BY-4.0. PMID: 35966206
Representative cortical layer 5/6 pyramidal neurons from control, (B) active MS lesion, (C) NAGM, (D) inactive MS lesion tissues immunolabelled with anti-SMI32, and their AIS immunolabelled with anti-AnkG. White rectangles in the merged channel panels indicate magnification areas and are presented in the lower panels. Dashed lines indicate the contour of the soma, arrowheads indicate the start of the AIS, and white lines represent soma-AIS gaps. Image from publication CC-BY-4.0. PMID:36451656
Representative micrographs showing immunohistochemical labelling for SSt (green), the AIS marker AnkyrinG (AnkG, magenta; cat. 75-146, 1:100), and their overlap (merge). The SSt soma is indicated with an asterisk (*) and the start and end of the AIS localised to that IN indicated (arrows). Image from publication CC-BY-4.0. PMID:36658491
Representative image of Alexa 555 (AL555, red) dye-filled RGC labeled for the axon initial segment (AIS) scaffolding protein ankyrin-G (AnkG, cat. 75-146, 1:200; green). Annotations demonstrate the dimensions of AIS distance from soma and length which were quantified. Image from publication CC-BY-4.0. PMID:37051140
Representative maximal projection of a confocal image of endogenous AnkG (Cat. 75-146) staining in rat cortical neurons transfected with GFP with or without lithium stimulation. Image from publication CC-BY-4.0. PMID:36969554
Example images of ankG labeling (Cat. 75-146, 1:200; green) in hRGCs (orange) treated with vehicle (A) or colchicine (B). Image from publication CC-BY-4.0. PMID:37010860
Immunostaining of mouse sliced optic nerve labeling Ankyrin G (Cat. 75-146, orange, 1:125) and Caspr (red). Nuclei are stained with DAPI. Image was taken at the CRL Molecular Imaging Center, RRID:SCR_017852, supported by the Helen Wills Neuroscience Institute. Image kindly provided by Anna Elleman, UC Berkley.
Representative confocal images showing the morphology of DIV21 primary hippocampal neurons over-producing mCherry (blue; circle highlights the complexity in panel 2, i.e. the number of processes that cross the circle is representative of the number of processes per cell) as well as EGFP–DHFR (−MTS; green; top panel) or Su9-EGFP–DHFR (+MTS; green; top panel) in the presence or absence of 100 nM MTX. Axons were stained with ankyrin-G (Cat. 75-146, 1:400; magenta). CC-BY-4.0. PMID:37315917
CD59 (green) and ankyrin-G (AnkG, cat. 75-146; red) staining of WT murine teased fibers. Image from publication CC-BY-4.0. PMID:37875972
Immunofluorescence labeling of WT (left panels) and CD59 KO mouse(right panels) teased fibers show normal node structure by CD59 (green) and AnkG (Cat. 75.146; red). Image from publication CC-BY-4.0. PMID:37875972
Reconstructions of the AIS (red) pooled from 3 independent experiments in 2 week wild-type (n = 27), iso-TDP-43G298S (n = 31), TDP-43G298S (n = 32), and C9orf72-3 (n = 21) MNs based on AnkG (Cat. 75-146; red) immunofluorescence staining. Counterstained against CHR2-YFP (cyan). White arrows indicate start and end positionsImage from publication CC-BY-4.0. PMID: 38019651
Staining of axon initial segment (AIS) showing specific labeling of AnkyrinG (Cat. 75-146, 1:250; red) in human iPSC-derived WT neuron. Nuclei stained with DAPI (blue). Image kindly provided by Yufei Liu, University of California, San Diego.
Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
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Feline, Hamster, Human, Mouse, Non-Human Primate, Rat
ELISA, ICC, IHC, IP, WB
Mouse
490
550
594
650
Biotin
Carrier-Free

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SKU: 75-146

Volume: 100 µL
Price:
$394.00
Ships: 1-2 business days

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