Anti-Ancient ubiquitous protein 1 (AUP1) Antibody

Name: Anti-Ancient ubiquitous protein 1 (AUP1) Antibody
Brand: Biosensis
SKU: S-035-100
Price: 327.0 USD
Availability: InStock

Our Anti-Ancient ubiquitous protein 1 (AUP1) sheep polyclonal primary antibody detects bovine, human, rat, and sheep Ancient ubiquitous protein 1 (AUP1), and is whole serum. It is validated for use in IHC-Frozen, WB.

Species Reactivity

Bovine, Human, Rat, Sheep

Applications

IHC, WB

Host Species

Sheep

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Data Sheet

SKU: S-035-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Ancient ubiquitous protein 1 (AUP1)

Scientific Background

AUP1 contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain thay may be involved in binding ubiquitin-conjugating enzymes. The unprocessed precusor is of 476 amino acids in length and has an estimated molecular weight of 53 kDa. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the AUP1 family. SIMILARITY: Contains 1 CUE domain.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC, WB

Host Species

Sheep

Immunogen

A synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ancient ubiquitous protein 1 conjugated to Blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from bovine, mouse and rat.

Immunogen Species

Human

Species Reactivity

Bovine, Human, Rat, Sheep

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:500-1:6000
IHC: 1:500-1:6000

Application Details

IHC: Use at an amount of 10 µg/mL with an incubation time of 1-3 days at 4ºC. This antiserum works in paraffin and 4% PFA fixed frozen sections. Penetration is the key to success. Over-fixed tissue is problematic. Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for Western blotting.

Conjugate

Unconjugated

Specificity

This antiserum is specific for ancient ubiquitous protein 1. This antibody is known to react with bovine, sheep, human and rat ancient ubiquitous protein 1. Other species have not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

AUP1

UniProt ID

Q9Y679

Shipping

25°C (ambient)

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Anti-Ancient ubiquitous protein 1 (AUP1) Antibody

SKU: S-035-100

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