Anti-Akt (N-terminal region) Antibody (M101)

Name: Anti-Akt (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: AM1011
Price: 285.0 USD
Availability: InStock

4 Citations

Our Akt (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Akt (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).

Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).

Western blot image of HEK293 cells treated with 100 nM insulin for 15, 30, 60, and 120 minutes measuring AKT activation between phosphorylated and total AKT protein levels. (Cat. AM1011, 1:1000). CC-BY-4.0 PMID: 37108445

Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: AM1011

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Akt (N-terminal region)

Scientific Background

Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M101

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

AKT1

Molecular Weight

Immunogen

Clone M101 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:250

Conjugate

Unconjugated

Specificity

The antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of phoshorylated Akt on SDS-PAGE immunoblots of A431 + calyculin A cell lysate. Similar results were seen in calyculin A treated human aortic endothelial and HeLa cells, rabbit spleen fibroblasts, and rat pituitary cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

PKBalpha, PKB, AKT

UniProt ID

P31749

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Rat

Western Blot: Human
PMID	Dilution	Publication
37108445	1:1000	Cimini, FA, et al. 2023. Dynamic Changes of BVRA Protein Levels Occur in Response to Insulin: A Pilot Study in Humans. International Journal of Molecular Sciences, 7282.
30941654	not listed	Hu, C, et al. 2019. Resveratrol inhibits the proliferation of estrogen receptor-positive breast cancer cells by suppressing EZH2 through the modulation of ERK1/2 signaling. Cell biology and toxicology, 445-456.

Western Blot: Rat
PMID	Dilution	Publication
38004105	1:1000	Rodrigues Junior, CF, et al. 2023. Changes in Skeletal Muscle Protein Metabolism Signaling Induced by Glutamine Supplementation and Exercise. Nutrients, 4711.
19880666	not listed	Wei, H, et al. 2010. Ischemic preconditioning and heat shock activate Akt via a focal adhesion kinase-mediated pathway in Langendorff-perfused adult rat hearts. American journal of physiology. Heart and circulatory physiology, H152-157.

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Anti-Akt (N-terminal region) Antibody (M101)

SKU: AM1011

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Akt (N-terminal region) Antibody

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