Anti-Actin (C-terminal region) Antibody (M202)

Name: Anti-Actin (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: AM2021
Price: 285.0 USD
Availability: InStock

4 Citations

Our Actin (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Actin (C-terminal region) and is Protein G purified. It is great for use in WB, ICC, IHC.

Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).

Formalin fixed, citric acid treated parafin sections of E18 mouse skeletal muscle. Sections were probed with anti-Actin (AM2021) then anti-Mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: AM2021

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Actin (C-terminal region)

Scientific Background

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation

Format

Protein G Purified

Clonality

Monoclonal

Clone

M202

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

ACTB

Molecular Weight

Immunogen

Clone (M202) was generated from a sequence corresponding to amino acids in the C-terminal region of human β-actin*. This human actin sequence is highly conserved in most eukaryotic actin isoforms.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:50
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of human A431, SYF, and HUVEC cells, as well as mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

UniProt ID

P60709

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
30147856	1:100	Barnes, J, et al. 2018. Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells. Molecular autism, 44.

Immunocytochemistry: Rat
PMID	Dilution	Publication
24362679	not listed	Muirhead, G, et al. 2014. The expression of neuronal sorting nexin 8 (SNX8) exacerbates abnormal cholesterol levels. Journal of Molecular Neuroscience: MN, 125-134.

Western Blot: Mouse
PMID	Dilution	Publication
24911000	not listed	Pritchard, AJ, et al. 2014. Fingolimod attenuates splenocyte-induced demyelination in cerebellar slice cultures. PloS One, e99444.

Western Blot: Rat
PMID	Dilution	Publication
24749734	1:500	Dutta, P, et al. 2014. The protein interacting with C-kinase (PICK1) interacts with and attenuates parkin-associated endothelial-like (PAEL) receptor-mediated cell death. Journal of Neurochemistry, 360-373.
24362679	not listed	Muirhead, G, et al. 2014. The expression of neuronal sorting nexin 8 (SNX8) exacerbates abnormal cholesterol levels. Journal of Molecular Neuroscience: MN, 125-134.

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Anti-Actin (C-terminal region) Antibody (M202)

SKU: AM2021

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Actin (C-terminal region) Antibody

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