Anti-Tyrosine Kinase Receptor A (TrkA) Antibody

Name: Anti-Tyrosine Kinase Receptor A (TrkA) Antibody
Brand: Biosensis
SKU: R-152-100
Price: 337.0 USD
Availability: InStock

Our Anti-Tyrosine Kinase Receptor A (TrkA) rabbit polyclonal primary antibody detects human, mouse, and rat Tyrosine Kinase Receptor A (TrkA), and is whole serum. It is validated for use in ELISA, IHC-Frozen.

Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat spinal cord (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat trigeminal nerve (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

Western blot of TrkA receptor in human neuroblastoma SH-SY5Y cell lysate (30 µg/lane). R-152-100 detects TrkA full-length protein at 130-140 kDa under non-reducing conditions (no DTT or beta-mercaptoethanol!). Higher molecular-weight bands may represent homodimeric forms of TrkA or heterodimeric TrkA-receptor complexes. The identity of lower molecular weight bands is unknown. SDS-PAGE: denatured and non-reducing; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 4°C (1/2500 dilution); Secondary antibody: anti-rabbit-HRP (1/6000) 2 hours at RT; Detection: Chemiluminiscence. Predicted MW of humanTrkA based on amino acid sequence: 84 kDa. The observed MW differs due to post-translational modification, mainly glycosylation.

Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat spinal cord (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IHC

Host Species

Rabbit

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Data Sheet

SKU: R-152-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Tyrosine Kinase Receptor A (TrkA)

Scientific Background

TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, IHC

Host Species

Rabbit

Immunogen

Extracellular domain of glycosylated human TrkA protein produced in mammalian cells was used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:1000-1:2500
IHC: 1:1000
ELISA: 1:5000

Conjugate

Unconjugated

Specificity

Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons. Reacts with human, rat and mouse TrkA. Other species not yet tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

MTC; Tropomyosin-related kinase receptor; TRK1; High affinity nerve growth factor receptor; TRKA; Neurotrophic tyrosine kinase receptor type 1; Trk-A; TRK1 transforming tyrosine kinase protein; p140-TrkA; Oncogene TRK; NTRK1; TRK1-transforming tyrosine kinase protein; TRK; gp140trk; neurotrophic tyrosine kinase; receptor; type 1; tropomyosin-related kinase A; tyrosine kinase receptor A

UniProt ID

P04629
UniProt Summary: Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand. Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.

NCBI Gene ID

4914

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Anti-Tyrosine Kinase Receptor A (TrkA) Antibody

SKU: R-152-100

Product Details

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