Anti-Tau (Ser416) Antibody

Name: Anti-Tau (Ser416) Antibody
Brand: PhosphoSolutions
SKU: p1573-416
Price: 456.0 USD
Availability: InStock

Our Anti-Tau (Ser416) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Tau (Ser416) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Western blot of rat brain homogenate showing specific immunolabeling of the ~59 kDa, ~65 kDa, ~68 kDa Tau isoforms phosphorylated at Ser416 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Immunostaining of FFPE human hippocampus-CA1 neurons labeling tau phosphorylated at Ser 416 (Cat. p1573-416, 1:200, red). The bright red stain labels the phosphorylated neurons while the thin, thread like stain labels the dystrophic neurites identifying the characteristic lesions of Alzheimer diseased tissue. Photo courtesy of Benecia Hong-Goka, Indiana Univ. SOM)

Western blot of rat brain homogenate showing specific immunolabeling of the ~59 kDa, ~65 kDa, ~68 kDa Tau isoforms phosphorylated at Ser416 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1573-416

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Tau (Ser416)

Scientific Background

Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Ser-416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Rabbit

Gene Name

MAPT

Molecular Weight

59/65/68 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 of rat tau, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate

Antibody Registry ID

AB_2492257

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:200

Conjugate

Unconjugated

Specificity

Specific for ~59, 65, 68 kDa tau protein phosphorylated at Ser416. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser416

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

AI413597; AW045860; DDPAC; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MAPT; MAPTL; MGC134287; MGC138549; MGC156663; Microtubule associated protein tau; Microtubule associated protein tau isoform 4; Microtubule-associated protein tau; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; Paired helical filament-tau; PHF tau; PHF-tau; PPND; PPP1R103; Protein phosphatase 1 regulatory subunit 103; pTau; RNPTAU; TAU; TAU_HUMAN; Tauopathy and respiratory failure included

UniProt ID

P19332
UniProt Summary: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. {ECO:0000269|PubMed:21985311, ECO:0000269|PubMed:32961270}.

NCBI Gene ID

4137

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Binder, L.I., Frankfurter, A. and Rebhun, L.I., 1985. The distribution of tau in the mammalian central nervous system. The Journal of Cell Biology, 101(4), pp.1371-1378. PMID: 3930508
Lee, V.M., 1995. Disruption of the cytoskeleton in Alzheimer's disease. Current Opinion in Neurobiology, 5(5), pp.663-668. PMID: 8580719
Goedert, M. and Jakes, R., 1990. Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization. The EMBO Journal, 9(13), pp.4225-4230. PMID: 2124967
Steiner, B., Mandelkow, E.M., Biernat, J., Gustke, N., Meyer, H.E., Schmidt, B., Mieskes, G., Söling, H.D., Drechsel, D. and Kirschner, M.W., 1990. Phosphorylation of microtubule‐associated protein tau: identification of the site for Ca2 (+)‐calmodulin dependent kinase and relationship with tau phosphorylation in Alzheimer tangles. The EMBO Journal, 9(11), pp.3539-3544. PMID: 2120043

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Anti-Tau (Ser416) Antibody

SKU: p1573-416

Product Details

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