Anti-Synphilin-1 (Sph1) Antibody

Name: Anti-Synphilin-1 (Sph1) Antibody
Brand: Biosensis
SKU: R-116-100
Price: 337.0 USD
Availability: InStock

Our Anti-Synphilin-1 (Sph1) rabbit polyclonal primary antibody detects human and rat Synphilin-1 (Sph1), and is whole serum. It is validated for use in IHC-Frozen.

Immunohistochemical staining of synphilin-1 positive Lewy bodies in dorsal rache nucleus in a case of Parkinson's disease using rabbit polyclonal antibody to human synphilin-1, catalogue number R-116-100.

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Species Reactivity

Human, Rat

Applications

IHC

Host Species

Rabbit

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Data Sheet

SKU: R-116-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Synphilin-1 (Sph1)

Scientific Background

Synuclein alpha interacting protein (Synphilin-1) contains several protein-protein interaction domains and interacts with alpha synuclein in neurons. Mutations of SNCAIP have been linked to Parkinson disease. The amino acid sequence of synphilin-1 shares a high level of identity with its human counterpart, particularly in regions containing ankyrin-like motifs and the coiled-coil domain. Expression pattern of synphilin-1 in tissues is similar in both mouse and human. Synphilin-1 has an important role in the formation of aggregates and cytotoxicity in Parkinson disease and also Dorfin may be involved in the pathogenic process by ubiquitylation of synphilin-1.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC

Host Species

Rabbit

Immunogen

A synthetic peptide (SLELNGEKDKDKGRTLQRT) as part of human synphilin-1a conjugated to KLH has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

IHC: 1:1000-1:2000

Application Details

This antiserum will stain Lewy bodies in Dorsal raphe nucleus of Parkinson's diseased human brain.

Conjugate

Unconjugated

Specificity

A high level of specificity has been shown for this antiserum by IHC on human brain. This antiserum is known to react with rat and human synphilin-1a.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

SYPH1; Synphilin-1a protein; synphilin-1; synuclein alpha interacting protein; Synphilin 1; SNCAIP

UniProt ID

Q6L986
UniProt Summary: Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
Miscellaneous
Constructs encoding portions of SNCA and SNCAIP co-transfected in mammalian cells promote cytosolic inclusions resembling the Lewy bodies of Parkinson disease. Coexpression of SNCA, SNCAIP, and PRKN result in the formation of Lewy body-like. ubiquitin-positive cytosolic inclusions. SNCAIP isoform 2 is particularly aggregation-prone. Familial mutations in PRKN disrupt the ubiquitination of SNCAIP and the formation of the ubiquitin-positive inclusions. These results provide a molecular basis for the ubiquitination of Lewy body-associated proteins and link PRKN and SNCA in a common pathogenic mechanism through their interaction with SNCAIP.

NCBI Gene ID

9627

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Anti-Synphilin-1 (Sph1) Antibody

SKU: R-116-100

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