Anti-SMAD7 (N-terminal region) Antibody

Name: Anti-SMAD7 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP4901
Price: 292.0 USD
Availability: InStock

Our SMAD7 (N-terminal region) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rat, and Xenopus SMAD7 (N-terminal region) and is Protein G purified. It is great for use in WB, ICC.

Western blot analysis of SMAD7 in human HepG2 cells. The blot was probed with rabbit polyclonal SMAD7 antibody (SP4901) at 1:250.

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Species Reactivity

Frog, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: SP4901

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

SMAD7 (N-terminal region)

Scientific Background

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Format

Protein G Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

SMAD7

Molecular Weight

Immunogen

This antibody was raised against amino acids from the N-terminal region of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.

Immunogen Species

Human

Species Reactivity

Frog, Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.05% BSA and 0.05% NaN3

Dilution Ranges

WB: 1:500
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects SMAD7 at 46 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CRCS3; MADH7; MADH8; mothers against decapentaplegic homolog 7; MAD homolog 8; SMAD; mothers against DPP homolog 7; hSMAD7; mothers against DPP homolog 8; MAD7

UniProt ID

O15105
UniProt Summary: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

NCBI Gene ID

4092

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Anti-SMAD7 (N-terminal region) Antibody

SKU: SP4901

Product Details

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