Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody

Name: Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody
Brand: Biosensis
SKU: S-079-100
Price: 347.0 USD
Availability: InStock

Our Anti-pro-Brain-derived neurotrophic factor (proBDNF) sheep polyclonal primary antibody detects human, mouse, and rat pro-Brain-derived neurotrophic factor (proBDNF), and is whole serum. It is validated for use in ELISA, Neutralization, WB.

Western blot analysis of proBDNF on DRG homogenate. lane 1: brain; lane 2: DRG. The antibody recognises monomer and dimer of the recombinant proBDNF.

Immunofluorescence analysis of proBDNF expression in human SHSY-5Y cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) SHSY-5Y cells were incubated with proBDNF antibody S-079-100 (1:1000, red) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-sheep-PE antibody (1:100, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). BDNF-IR is observed in the perinuclear cytoplasm. Magnification: 100x.

Separation and detection of proBDNF in conditioned 293 cell culture medium (Lanes 1 and 3, 50 ng/lane) and purified mature BDNF (Lanes 2 and 4, 50 ng/lane) by reduced SDS-PAGE and western blotting. Anti-proBDNF sheep antibody S-079-100 (1/1000) detects 2 bands (Lane 1) with the apparent molecular weight of ≈ 32 kDa (red arrow) and ≈ 64 kDa (blue arrow) corresponding to glycosylated proBDNF monomer and dimer, respectively. S-079-100 is specific for proBDNF and does not recognize mature BDNF (Lane 2). Rabbit anti-BDNF antibody R-1707-100 (0.2 µg/mL) is a superb antibody for western blotting. It detects mature BDNF (≈ 14 kDa, Lane 4, black arrow) and shows lower affinity for pro-BDNF monomer (Lane 3, red arrow) and proBDNF dimer (Lane 3, blue arrow).

Western blot analysis of proBDNF on DRG homogenate. lane 1: brain; lane 2: DRG. The antibody recognises monomer and dimer of the recombinant proBDNF.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Neutralize, WB

Host Species

Sheep

Go to Full Product Details

Data Sheet

SKU: S-079-100

Volume/Size: 100 µL

Variant

Price:

$347.00

Quantity:

Ships: 1-2 business days

Product Details

Target

pro-Brain-derived neurotrophic factor (proBDNF)

Scientific Background

Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner. This antibody is raised in sheep to detect the prodomain of BDNF and not the mature peptide.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, Neutralize, WB

Host Species

Sheep

Immunogen

The recombinant prodomain fragment of human brain-derived neurotrophic factor

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Conjugate

Unconjugated

Specificity

Confirmed to react with purified human proBDNF, crossreact with mouse and rat proBDNF Cross reactivity with other species than human, mouse and rat has not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

ANON2; Proform brain derived neurotrophic factor; abrineurin; neurotrophin; brain-derived neurotrophic factor

UniProt ID

P23560
UniProt Summary: Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

NCBI Gene ID

627

Shipping

25°C (ambient)

Recover password

Create my account

Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody

SKU: S-079-100

Product Details

Bulk Order Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody

Related Products

Recently Viewed