Anti-PERK (phosphorylated and non phosphorylated) Antibody

Name: Anti-PERK (phosphorylated and non phosphorylated) Antibody
Brand: Biosensis
SKU: R-1333-100
Price: 347.0 USD
Availability: InStock

Our Anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) rabbit polyclonal primary antibody detects mouse PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK), and is whole serum. It is validated for use in ICC, WB.

Western Blot using mouse cultured astrocyte extracts at rest (Cntrl) and after 60 mins of ER stress induction by oxygen glucose deprivation (OGD). The left panel membrane was probed with Rabbit polyclonal antibody to mouse PERK: Whole serum (R-1333-100). The right membrane was similarly probed in combination with the antigenic peptide used to generate the antibody. Image taken from Bollo et al (2010) Figure S4.

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Species Reactivity

Mouse

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1333-100

Volume/Size: 100 µL

Variant

Price:

$347.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK)

Scientific Background

PERK (PKR-like ER kinase) is a single-pass type I ER membrane protein with a stress-sensing luminal domain connected by a transmembrane segment to a cytoplasmic-kinase domain.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ICC, WB

Host Species

Rabbit

Immunogen

A recombinant peptide from mouse PERK.

Immunogen Species

Mouse

Species Reactivity

Mouse

Storage

Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Liquid

Buffer

50% glycerol

Dilution Ranges

WB: 1:500
IP: 60 µg/mL

Conjugate

Unconjugated

Specificity

This antiserum is known to recognize both phosphorylated and non phosphorylated mouse PERK.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Pek; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; pancreatic eIF2-alpha kinase; Eif2ak3; Perk

UniProt ID

Q9Z2B5
UniProt Summary: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress, such as unfolded protein response (UPR). Key effector of the integrated stress response (ISR) to unfolded proteins: EIF2AK3/PERK specifically recognizes and binds misfolded proteins, leading to its activation and EIF2S1/eIF-2-alpha phosphorylation. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. The EIF2AK3/PERK-mediated unfolded protein response increases mitochondrial oxidative phosphorylation by promoting ATF4-mediated expression of COX7A2L/SCAF1, thereby increasing formation of respiratory chain supercomplexes. In contrast to most subcellular compartments, mitochondria are protected from the EIF2AK3/PERK-mediated unfolded protein response due to EIF2AK3/PERK inhibition by ATAD3A at mitochondria-endoplasmic reticulum contact sites. In addition to EIF2S1/eIF-2-alpha, also phosphorylates NFE2L2/NRF2 in response to stress, promoting release of NFE2L2/NRF2 from the BCR(KEAP1) complex, leading to nuclear accumulation and activation of NFE2L2/NRF2. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

NCBI Gene ID

13666

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2-8°C (on cold packs)

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Anti-PERK (phosphorylated and non phosphorylated) Antibody

SKU: R-1333-100

Product Details

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