Anti-Ku80 Antibody

Name: Anti-Ku80 Antibody
Brand: PhosphoSolutions
SKU: 24368
Price: 456.0 USD
Availability: InStock

Our Ku80 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects hamster, human, mouse, and rat Ku80 and is antigen affinity purified. It is great for use in WB.

Immunoprecipitation showing detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.

Western Blot of 50 ug lysates of A431 (lane 1) and AR42J (lane 2) cells showing specific immunolabeling of Ku80.

Western Blot of MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane) showing specific immunolabeling of Ku80.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~83 kDa Ku-80 protein.

Immunoprecipitation showing detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.

Click on image to zoom

Species Reactivity

Hamster, Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: 24368

Title: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ku80

Scientific Background

Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

XRCC5

Immunogen

Synthetic peptide corresponding to amino acids from an internal sequence of the human Ku80 protein

Immunogen Species

Human

Species Reactivity

Hamster, Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.02% NaN3

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000

Specificity

Reacts with the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

KARP-1; XRCC5; KU80; KUB2; Ku86; NFIV; X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen; 80kDa; Ku86 autoantigen related protein 1; TLAA; lupus Ku autoantigen protein p86; nuclear factor IV; thyroid-lupus autoantigen; G22P2

UniProt ID

P13010
UniProt Summary: DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC6 (Ku70), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs. This activity cleans the termini of abasic sites, a common form of nucleotide damage, preparing broken ends for ligation. It may also possess 3'-5' DNA helicase activity, although this has not been confirmed in vivo, and its physiological significance remains unclear. Beyond DNA repair, the protein contributes to telomere maintenance. It is also implicated in transcriptional regulation, acting as a cofactor for various transcription factors. It plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. Can also bind RNAs and recruits PRKDC to a wide range of cellular RNAs, including the U3 small nucleolar RNA, playing a role in the biogenesis of ribosomal RNAs.

NCBI Gene ID

7520

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Anti-Ku80 Antibody

SKU: 24368

Product Details

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