Anti-FBXW7 (hCdc4) Antibody

Name: Anti-FBXW7 (hCdc4) Antibody
Brand: PhosphoSolutions
SKU: 24253
Price: 456.0 USD
Availability: InStock

Our FBXW7 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human FBXW7 and is antigen affinity purified. It is great for use in WB.

Species Reactivity

Human

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: 24253

Title: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

FBXW7

Scientific Background

Cyclin E, one of the activators of the cyclin-dependent kinase Cdk2, is critical for the initiation of DNA replication and other S-phase functions. The proper timing and amplitude of cyclin E expression seem to be important, because elevated levels of cyclin E have been associated with a variety of malignancies and constitutive expression of cyclin E leads to genomic instability. Phosphorylated cyclin E depends on the novel human gene, hCdc4, that was found to be mutated in a cell line derived from breast cancer that expressed extremely high levels of cyclin E.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

FBXW7

Immunogen

Synthetic peptide corresponding to amino acids from the N-terminal region of human Cdc4 protein

Immunogen Species

Human

Species Reactivity

Human

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.02% NaN3

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000

Specificity

Reacts with the N terminus of the 69 kDa isoform of the hCdc4 protein

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

F-box protein FBX30; Cdc4; AGO; FBW6; FBW7; FBX30; FBXO30; FBXW6; SEL-10; SEL10; hAgo; hCdc4; F-box/WD repeat-containing protein 7; F-box protein FBW7; F-box protein SEL-10; archipelago; homolog of C elegans sel-10; FBXW7; F box and WD 40 domain protein 7

UniProt ID

Q969H0
UniProt Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. Involved in bone homeostasis and negative regulation of osteoclast differentiation. Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination. Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage. The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining.

NCBI Gene ID

55294

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Anti-FBXW7 (hCdc4) Antibody

SKU: 24253

Product Details

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