Anti-EphrinB (Tyr317) Antibody

Name: Anti-EphrinB (Tyr317) Antibody
Brand: PhosphoSolutions
SKU: p1110-317
Price: 456.0 USD
Availability: InStock

Our Anti-EphrinB (Tyr317) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat EphrinB (Tyr317) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr317 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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Species Reactivity

Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1110-317

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

EphrinB (Tyr317)

Scientific Background

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr-298) plays a key role in EphrinB signaling (Kalo, et al., 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

EPHB2

Molecular Weight

46 kDa

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH). Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.

Immunogen Species

Chicken

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Human, Xenopus, Zebrafish

Antibody Registry ID

AB_2492087

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr317. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr317

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CEK5 ligand; EFNB1; ELK L; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B1; Ephrin B2; LERK2

UniProt ID

P28693
UniProt Summary: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation (PubMed:30213874). {ECO:0000269|PubMed:15300251, ECO:0000269|PubMed:30213874}.

NCBI Gene ID

396513

Shipping

Blue Ice

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Anti-EphrinB (Tyr317) Antibody

SKU: p1110-317

Product Details

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