Anti-Ephexin-1 (Tyr-87), Phosphospecific Antibody

Name: Anti-Ephexin-1 (Tyr-87), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EP2841
Price: 340.0 USD
Availability: InStock

1 Citation

Our Ephexin-1 (Tyr-87) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Ephexin-1 (Tyr-87) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Ephexin-1 (C-terminal region) (lanes 1 & 2) and anti-Ephexin-1 (Tyr-87) (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal Ephexin-1 (C-terminal region) and Ephexin-1 (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Ephexin-1 (C-terminal region) (lanes 1 & 2) and anti-Ephexin-1 (Tyr-87) (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: EP2841

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ephexin-1 (Tyr-87)

Scientific Background

The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

NGEF

Molecular Weight

Immunogen

Phospho-ephexin-1 (Tyr-87) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 87 in mouse ephexin-1. This sequence has significant homology to the conserved region in human and rat ephexin-1.

Immunogen Species

Mouse

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects an 80 kDa* band corresponding to ephexin-1 in Western blots of human A431 treated with pervanadate. This band is removed by alkaline phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-87

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

EphA, Eph, NGEF

UniProt ID

Q8CHT1

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Chicken
Western Blot: Chicken

Immunocytochemistry: Chicken
PMID	Dilution	Publication
30237102	not listed	Fiore, L., et al. 2019. Regulation of axonal EphA4 forward signaling is involved in the effect of EphA3 on chicken retinal ganglion cell axon growth during retinotectal mapping. Experimental Eye Research, 46-60.

Western Blot: Chicken
PMID	Dilution	Publication
30237102	not listed	Fiore, L., et al. 2019. Regulation of axonal EphA4 forward signaling is involved in the effect of EphA3 on chicken retinal ganglion cell axon growth during retinotectal mapping. Experimental Eye Research, 46-60.

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Anti-Ephexin-1 (Tyr-87), Phosphospecific Antibody

SKU: EP2841

Product Details

Product Specific References for Applications and Species

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