Anti-Apolipoprotein E (ApoE) Antibody

Name: Anti-Apolipoprotein E (ApoE) Antibody
Brand: Biosensis
SKU: G-1784-100
Price: 337.0 USD
Availability: InStock

Our Anti-Apolipoprotein E (ApoE) goat polyclonal primary antibody detects human Apolipoprotein E (ApoE), and is affinity-purified. It is validated for use in ELISA.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA

Host Species

Goat

Go to Full Product Details

Data Sheet

SKU: G-1784-100

Volume/Size: 100 µg

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Apolipoprotein E (ApoE)

Scientific Background

Apolipoprotein E (ApoE) is a lipoprotein involved in fat metabolism and acts as cholesterol carrier between cells and across tissues. On a genetic level, three APOE alleles are described, APOE2, APOE3 and APOE4. These alleles give rise to six APOE isoforms, which are differentially implicated in various diseases. In the peripheral system, APOE4 is linked to increased risk of atherosclerosis. In the CNS, the ability of APOE4 in clearing beta-amyloid is impaired, while APOE3 and APOE2 are more efficient in performing this task. The APOE4 genotype in particular has been linked to increased risk for developing Alzheimer's Disease.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA

Host Species

Goat

Immunogen

Recombinant human Apolipoprotein E

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C to -80°C for higher stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from a solution containing 50 mM Tris, pH 7.5, 0.4 M NaCl, 0.01 M EDTA, 0.1% trehalose, 0.07% sodium azide.

Dilution Ranges

ELISA: 0.1-1 µg/mL

Application Details

ELISA (0.1-1 µg/mL). Other applications not tested as yet.

Conjugate

Unconjugated

Specificity

Human. Species cross-reactivity not tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

AD2; APOE; ApoE4; LDLCQ5; LPG; apolipoprotein E; apolipoprotein E3

UniProt ID

P02649
UniProt Summary: APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE also has a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis. First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues. Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes. APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting. APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells. Binds to the immune cell receptor LILRB4. APOE may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP.

NCBI Gene ID

348

Shipping

25°C (ambient)

Recover password

Create my account

Anti-Apolipoprotein E (ApoE) Antibody

SKU: G-1784-100

Product Details

Bulk Order Anti-Apolipoprotein E (ApoE) Antibody

Related Products

Recently Viewed