Anti-Sphingosine Kinase 2 (Thr-578), Phosphospecific Antibody

Name: Anti-Sphingosine Kinase 2 (Thr-578), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP4631
Price: 340.0 USD
Availability: InStock

4 Citations

Our Sphingosine Kinase 2 (Thr-578) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Sphingosine Kinase 2 (Thr-578) and is antigen affinity purified. It is great for use in WB.

Western blot of human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-SK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-SK2 (Thr-578) (lanes 3, 4, 7, & 8).

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Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

SKU: SP4631

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Sphingosine Kinase 2 (Thr-578)

Scientific Background

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. Regulation of SK1 and SK2 activity may occur through phosphorylation. SK1 is phosphorylated at Ser-225 by ERK leading to increased activity and translocation to the plasma membrane. SK2 is phosphorylated in response to EGF, PKC activators, and phorbol esters. ERK1 can phosphorylate both Ser-351 and Thr-578, and non-phosphorylatable mutants of these sites suppress ERK1-mediated chemotaxis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

SPHK2

Molecular Weight

Immunogen

Phospho-SK2 (Thr-578) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-578 in human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and is not well conserved in SK1.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects 70 kDa* proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-578

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SK2, Spk2, Sphk2

UniProt ID

Q9NRA0

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
27864331	1:100	Fu, P, et al. 2016. Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium. The journal of biological chemistry, 27187-27203.

Western Blot: Human
PMID	Dilution	Publication
35178477	not listed	Dai, L, et al. 2022. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation. Open medicine, 229-238.
33525436	not listed	Bernacchioni, C, et al. 2021. Role of Sphingosine 1-Phosphate Signalling Axis in Muscle Atrophy Induced by TNFα in C2C12 Myotubes. International journal of molecular sciences, 1280.

Western Blot: Mouse
PMID	Dilution	Publication
24686171	not listed	Wallington-Beddoe, CT, et al. 2014. Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression. Cancer research, 2803-2815.

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Anti-Sphingosine Kinase 2 (Thr-578), Phosphospecific Antibody

SKU: SP4631

Product Details

Product Specific References for Applications and Species

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