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Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658). The latter shows no detection of PKCα after phosphatase treatment.Formalin fixed, citric acid treated parafin sections of adult mouse brain. Sections were probed with anti-PKCα (PM2371) then anti-mouse:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-PKCα (Central region) Antibody
$370
Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI3 Kinase p85 (C-terminal region) antibody (PM2531), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PI3 Kinase, p85 (C-terminal region) Antibody
$370
Bar graph showing rabbit polyclonal anti-Phosphotyrosine (PP2221) binding to a variety of phosphotyrosine containing peptides, but no binding to unphosphorylated peptide (beta-Catenin (a.a. 29-45).Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Phosphotyrosine Antibody
$285
Western blot of HeLa cells treated with pervanadate (1 mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Phosphotyrosine Antibody
$285
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. The blot was untreated (lane 1) or treated with lambda phosphatase (lanes 2), then probed with anti-Phosphothreonine (PP4641) at 1:1000.Immunocytochemical labeling of phosphothreonine upregulation in control (left) or calyculin A-treated HeLa cells (right). The cells were labeled with rabbit polyclonal anti-Phosphothreonine (PP4641). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-Phosphothreonine Antibody
$285
Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).Immunocytochemical labeling of PD-L1 in aldehyde fixed human MDA-MB-231 carcinoma cells untreated (A) or treated with 100 ng/ml IFNγ for 48 hrs (B). The cells were labeled with mouse monoclonal anti-PD-L1 (PM0801). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-PD-L1 (Extracellular region) M080 Antibody
$370
Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).Immunocytochemical labeling of PD-L1 in aldehyde fixed human MDA-MB-231 carcinoma cells untreated (A) or treated with 100 ng/ml IFNγ for 48 hrs (B). The cells were labeled with mouse monoclonal anti-PD-L1 (PM0511). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-PD-L1 (Extracellular region) M051 Antibody
$370
Western blot of MDA-MB-435 cells untreated (lanes 1 and 3) or treated with EGF (100 ng/ml) for 1 hr (lanes 2 & 4). Blots were probed with mouse monoclonal anti-PDK1 (C-terminal region) or rabbit polyclonal anti-PDK1 (Ser-241).
PhosphoSolutions Anti-PDK1 (Ser-241), Phosphospecific Antibody
$340
Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK1 (C-terminal region) mouse monoclonal antibody or anti-PDK1 (Tyr-9) (PP1431) rabbit polyclonal antibody.Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PDK1 (Tyr-9), Phosphospecific Antibody
$340
Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK1 (C-terminal region) (PM1461).Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PDK1 (C-terminal region) Antibody
$285
Western blot image of A431 cells untreated (lanes 1 and 3) or treated with pervanadate (lanes 2, 4, 5 & 6). Blots were probed with anti-PDK1 (PP1411) or anti-PDK1 (Tyr-9) (PP1431). The latter was used in the presence of no peptide (lane 4), phospho-PDK1 (Tyr-9) peptide (lane 5), or an unrelated phosphotyrosine peptide (lane 6).Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PDK1 (N-terminus) Antibody
$285
Western blot analysis of human A431 cells treated with Calyculin A (100 nm) for 30 min (lanes 1-6). The blot was treated with lambda phosphatase (lanes 2, 4, & 6), then probed with rabbit polyclonal anti-Paxillin (C-terminal; PP1161) (lanes 1 & 2), anti-phospho-Paxillin (Thr-538; PP4491) (lanes 3 & 4), and mouse monoclonal anti-Paxillin (PM1071) (lanes 5 & 6).
PhosphoSolutions Anti-Paxillin (Thr-538), Phosphospecific Antibody
$340
Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).
PhosphoSolutions Anti-Paxillin (Ser-178), Phosphospecific Antibody
$340
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Paxillin (Ser-83), Phosphospecific Antibody
$340
Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Paxillin (PM1071) or anti-phospho-Paxillin (Tyr-31) (PM1021).
PhosphoSolutions Anti-Paxillin (Tyr-31), Phosphospecific Antibody
$340
Western blot analysis of A431 cells. The blot was probed with rabbit polyclonal anti-Paxillin (PP1161) at 1:500 & 1:2000 (lanes 1 & 2) or with mouse monoclonal anti-Paxillin (PM1071) at 1:2000 & 1:5000 (lanes 3 & 4).
PhosphoSolutions Anti-Paxillin (C-terminal) Antibody
$285
Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Paxillin Antibody
$370
Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lanes 2 & 6) then probed with anti-PAK6 (N-terminal) (lanes 1-4) or anti-PAK6 (Ser-165) phospho-specific (lanes 5-8). The antibodies were used in the presence of unrelated (lane 3) and PAK6 (N-terminal) (lane 4) peptide or PAK6 (Ser-165) (lane 7) and unrelated phospho-serine (lane 8) peptides, respectively.
PhosphoSolutions Anti-PAK6 (Ser-165), Phosphospecific Antibody
$340
Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lanes 2 & 6) then probed with anti-PAK6 (N-terminal) (lanes 1-4) or anti-PAK6 (Ser-165) phospho-specific (lanes 5-8). The antibodies were used in the presence of unrelated (lane 3) and PAK6 (N-terminal) (lane 4) peptide or PAK6 (Ser-165) (lane 7) and unrelated phospho-serine (lane 8) peptides, respectively.
PhosphoSolutions Anti-PAK6 (N-terminal region) Antibody
$340
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).
PhosphoSolutions Anti-p130 Cas (Tyr-762), Phosphospecific Antibody
$340
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).
PhosphoSolutions Anti-p130 Cas (Tyr-751), Phosphospecific Antibody
$340
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).Immunocytochemical labeling of p130 Cas in aldehyde fixed human A431 epidermoid carcinoma cells. The cells were labeled with mouse monoclonal anti-p130 Cas (PM1441). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-p130 Cas (C-terminal region) Antibody
$370
A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38α (a.a. 319-328) (lanes 1 & 2) or anti-p38α (Tyr-323) (lanes 3 & 4).Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38α MAP Kinase (Tyr-323), Phosphospecific Antibody
$340
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
$370

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