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Immunohistochemistry of retinal tissue showing specific staining of Shank at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ASD1, and ASD2 conditions. The fluorescence intensity of Shank positive puncta was measured using antibodies specific for Shank1, Shank2, and Shank3. Exemplary images (upper panel) and quantification of average puncta signal intensity of 10 cells per condition (lower panel). Merged images show additional DAPI staining of the nucleus. Neurons grown under ASD1 conditions show a significant decrease of synaptic Shank proteins, while neurons
Western Blot of rat brain lysate showing specific immunolabeling of SHANK1.
Western Blot of transfected COS-7 cells showing specific immunolabeling of Acinus. This antibody recognizes both long and short forms of Acinus protein.
Western Blot of transfected COS-7 cell lysate showing specific immunolabeling of Acinus.
Western Blot of NIH3T3 cell lysate showing specific immunolableing of FLASH.
Western Blot of NIH3T3 cell lysate showing specific immunolabeling of FLASH
Western Blot of Interleukin-1-stimulated 293 cell lysate showing specific immunolabeling of IKAP.
Western Blot of Interleukin-1-stimulated 293 cells showing specific immunolabeling of IKAP.
Western Blot of stimulated HEK 293 cells showing specific immunolabeling of IKK-gamma in Interleukin 1.
Western Blot of Interleukin 1 stimulated 293 cell lysate showing specific immunolabeling of IKK-gamma.
Western Blot of testis lysate showing specific immunolabeling of FHL5.
Western Blot of brain lysate showing specific immunolabeling of GABA-B R1.
Western Blot of rat brain lysate showing specific immunolabeling of GABA-B R2.
Western Blot of rat brain lysate showing specific immunolabeling of the GABARAP.
Western Blot of CD4+ cells showing specific immunolabeling of CD28.
Western Blot of HeLa cell lysate showing specific immunolabeling of Alien (aka COP9 signalosome complex subunit 2).
Western Blot of human pancreas lysate showing specific immunolabeling of SFRP5.
Western Blot of mouse pancreas lysate showing specific immunolabeling of Frizzled-7.

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