Anti-c-FOS Antibody (2H2)

Our Anti-c-FOS primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat c-FOS and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (Cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (Cat. 309-cFOS, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunostaining of mouse brain labeling c-Fos protein in green (Cat. 309-cFOS, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells (Cat. 309-cFOS), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes. Image from publication CC-BY-4.0. PMID: 36732588
Immunolabeling of a section of rat brain that specifically labels c-FOS protein (Cat. 309-cFOS, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
(A, B) confocal microscope images showing PV, WFA, and c-Fos (Cat. 309-cFOS) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment. Image from publication CC-BY-4.0. PMID: 37855072.
Immunofluorescence of young male and female wild type (WT) and Alzheimer's Disease (AD) mouse brain labelling nucleus-specific c-Fos + cells in the DMH, VMH, ARH, TuN, and LH regions. Image from publication CC-BY-4.0. PMID: 39272160
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (Cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (Cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
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Bovine, Human, Mouse, Rat
ELISA, ICC, IHC, WB
Mouse

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SKU: 309-cFOS

Volume: 100 µL
Price:
$456.00
Ships: 1-2 business days

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