Monoclonal Antibodies

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Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Axl/UFO (Extracellular region) Antibody
$379
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).Immunocytochemical labeling of ATM phosphorylation in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal ATM (C-terminal region) (AM3611) and ATM (Ser-1981) (AM3661). The antibodies were detected using goat anti-mouse-DyLight® 594.
PhosphoSolutions Anti-ATM (Ser-1981), Phosphospecific Antibody
$348
Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 (N-terminus) Antibody
$292
PhosphoSolutions Anti-APC (Central region) Antibody
$292
Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-5).Western blot of A431 cell lysate only (lane 1), AM0171 antibody only (lane 2), and AM0171 antibody immunoprecipitate from A431(lane 3). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50 kDa, while Annexin A5 band is observed at 35 kDa.
PhosphoSolutions Anti-Annexin A5 Antibody
$379
Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2).Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Annexin A2 (C-terminal region) Antibody
$379
Western blot of human A431 (lane 1), MDA-MB-231 (lane 2), LNCaP (lane 3), MeWo (lane 4), HUVEC (lane 5), Jurkat (lane 6), K562 (lane 7), and PC3 (lane 8) cell lysates. The blot was probed with mouse monoclonal anti-Annexin A2 antibody (AM0091) at 1:500 (lanes 1-8).Immunocytochemical labeling of Annexin A2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Annexin A2 (clone M009). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Annexin A2 Antibody
$379
Western blot of human A431 cells (lane 1), A549 cells (lane 2), LNCaP cells (lane 3), full-length recombinant human annexin A1 (lane 4), and recombinant human annexin A2 (lane 5). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0211) at 1:1000 (lanes 1-5).Immunocytochemical labeling of Annexin A1 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-Annexin A1 (clone M021). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Annexin A1 Antibody
$379
Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M562 (lanes 1-6) at a dilution of 1:1,000.Immunocytochemical labeling of ALDH1A1 in aldehyde fixed and NP-40 permeabilized human A431 cells. The cells were labeled with mouse monoclonal anti-ALDH1A1 (AM5621). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-ALDH1A1 (N-terminal region) Antibody
$379
Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M558 (lanes 1-6) at a dilution of 1:1,000.
PhosphoSolutions Anti-ALDH1A1 (N-terminal region) Antibody
$348
Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).
PhosphoSolutions Anti-Akt (N-terminal region) Antibody
$292
Western blot analysis of mouse white adipose tissue (lanes 1 & 3) and human recombinant full length adiponectin (lanes 2 & 4). The blot was probed with mouse monoclonals anti-Adiponectin (C-terminal) antibody M577 (lanes 1 & 2) or anti-Adiponectin (C-terminal) antibody M576 (lanes 3 & 4).
PhosphoSolutions Anti-Adiponectin (C-terminal region) Antibody
$348
Western blot analysis of mouse white adipose tissue (lanes 1 & 3) and human recombinant full length adiponectin (lanes 2 & 4). The blot was probed with mouse monoclonals anti-Adiponectin (C-terminal) antibody M577 (lanes 1 & 2) or anti-Adiponectin (C-terminal) antibody M576 (lanes 3 & 4).
PhosphoSolutions Anti-Adiponectin (C-terminal region) Antibody
$348
Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).Formalin fixed, citric acid treated parafin sections of E18 mouse skeletal muscle. Sections were probed with anti-Actin (AM2021) then anti-Mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Actin (C-terminal region) Antibody
$292
Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-β-Actin (AM0081) at 1:1000 (lanes 1-6).Immunocytochemical labeling of β-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-β-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-β-Actin Antibody
$379
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).
PhosphoSolutions Anti-c-Abl (C-terminal region) Antibody
$292
Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).
PhosphoSolutions Anti-Progesterone Receptor (Ser294) Antibody
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Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
PhosphoSolutions Anti-Progesterone Receptor (Ser190) Antibody
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Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.
PhosphoSolutions Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody
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Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in green. Nuclei are visualized in blue with DAPI.
PhosphoSolutions Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody
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Immunofluorescence of a section of rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons and cell bodies and dendrites of Purkinje cells with alpha-II-spectrin (Cat. 98-A2SM, 1:2000, green) and labeling the processes of Bergmann glia and astrocytes with anti-GFAP (Cat. 621-GFAP, 1:5000, red). Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.
PhosphoSolutions Anti-Alpha II Spectrin Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.Immunostaining of HeLa cells showing strong mitochondrial labeling of HSP60 (Cat. 750-HSP60, 1:5000, red) and nuclear staining with DAPI (blue).
PhosphoSolutions Anti-Hsp60 Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX1 protein.Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of FOX1 (Cat. 581-FOX1, 1:500, green).
PhosphoSolutions Anti-FOX1 Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.Immunofluorescence of HeLa cells showing clear localized labeling of the DNA in the nucleus with Anti-EWS (Cat. 540-EWS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:5000, red) labels the cytoplasmic intermediate filament, and Hoechst DNA stain (blue).
PhosphoSolutions Anti-EWS Antibody
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