Monoclonal Antibodies

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Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38α (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).Immunocytochemical labeling of p38α in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38α (PM5481). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) Antibody
$292
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) M138 Antibody
$379
Western blot analysis of p21 expression in human endothelial cells (lanes 1 & 2). The blots were probed with mouse monoclonal anti-p21 at 1:250 (lane 1) and 1:1000 (lane 2).
PhosphoSolutions Anti-p21/CIP1/WAF1 Antibody
$292
Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Osteopontin (N-terminal region) Antibody
$348
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-nNOS (C-terminal region) Antibody
$379
Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1µg/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NP2131).
PhosphoSolutions Anti-iNOS (C-terminal region) Antibody
$292
Western blot analysis of human umbilical vein endothelial cells stimulated with Calyculin A and untreated (lanes 1, 3, 5) or treated (lanes 2, 4, 6) with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).
PhosphoSolutions Anti-eNOS (Ser-632), Phosphospecific Antibody
$348
Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NM2211) and rabbit polyclonal eNOS (NP2281) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-eNOS (C-terminal region) Antibody
$379
Western blot image of recombinant human Neuropilin-1 (lanes 1 & 3) and human PC3 cells (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with mouse monoclonal anti-Neuropilin-1 (lanes 3 & 4).
PhosphoSolutions Anti-Neuropilin-1 (a1 CUB Domain) Antibody
$348
Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), and brain (lane 6) tissues. The blot was probed with anti-Na+/K+ ATPase β3 (NM0251) at 1:1000.Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M025). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Na+/K+ ATPase β3 (Extracellular) Antibody
$379
Western blot of NM0161 immunoprecipitates (IP) and whole lysates. The NM0161 antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-Na+/K+ ATPase β3 NM0161 (lanes 1-7). The arrow designates native antibody, while the β3 subunit migrates around 40 kDa.Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Na+/K+ ATPase β3 (Extracellular) Antibody
$379
Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Mitofilin Antibody
$379
Native western blot image of human laminin isoforms: laminin 521 (α5β2γ1), laminin 121 (α1β2γ1), laminin 221 (α2β2γ1), laminin 332 (α3β3γ2), laminin 511 (α5β1γ1), laminin 411 (α4β1γ1), as well as human A431, A549, and NCI-H2052 cells. The blot was probed with mouse monoclonal anti-Laminin β2/γ1 subunit (LM0461) at 1:1000.Immunocytochemical labeling of laminin β2/γ1 subunits in aldehyde fixed and NP-40 permeabilized human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Laminin β2/γ1 subunits (LM0461). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Laminin β2/γ1 Subunits Antibody
$348
Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight® 594.Representative Standard Curve using mouse monoclonal antiL1CAM (LM0231) for ELISA capture of human recombinant L1CAM protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
PhosphoSolutions Anti-L1CAM (Extracellular) Antibody
$379
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-JNK1 (lanes 1 & 2) or anti-JNK1 (T183/Y185) (lanes 3 & 4).Immunocytochemical labeling of JNK in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal JNK (C-terminal region) (Left) or mouse monoclonal JNK (Thr-183/Tyr-185) (Right). The antibodies were detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-JNK (Thr-183/Tyr-185), Phosphospecific Antibody
$348
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-JNK1 (lanes 1 & 2) or anti-JNK1 (T183/Y185) (lanes 3 & 4).Immunocytochemical labeling of JNK in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal JNK (C-terminal region) (Left) or mouse monoclonal JNK (Thr-183/Tyr-185) (Right). The antibodies were detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-JNK1 (C-terminal region) Antibody
$379
Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-Integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin β4 (lanes 5 & 6).Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.
PhosphoSolutions Anti-Integrin β4 (Cytoplasmic region) Antibody
$379
Western blot analysis of human umbilical vein endothelial cells (HUVEC). The blots were probed with mouse monoclonal anti-integrin β3 (IM5811) at 1:250 (lane 1) and 1:1000 (lane 2).
PhosphoSolutions Anti-Integrin β3 (Extracellular region) Antibody
$348
Western blot analysis of native cell lysates from human NCI-H446 lung cancer cells (lane 1), LNCaP prostate cancer cells (lane 2), and MCF7 breast cancer cells (lane 3). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0411) at 1:1000 dilution.Immunocytochemical labeling of Integrin β1 in aldehyde fixed and NP40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (IM0411). The antibody was detected using goat anti-mouse Ig:DyLight® 594.
PhosphoSolutions Anti-Integrin β1 (Extracellular region) Antibody
$379
Western blot analysis of integrin β1 expression in native human cells and tissues: A549 (lane 1) LNCaP (lane 2), MDA-MB-231 (lane 3) breast (lane 4), lung (lane 5), and skin (lane 6). The blot was probed with mouse monoclonal anti-Integrin β1 (IM0321) at 1:1000 dilution.Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (IM0321). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Integrin β1 (Extracellular region) Antibody
$348
Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and MDA-MB-231 (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0061) at 1:1000 dilution.Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (clone M006). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Integrin β1 (Extracellular region) Antibody
$379
Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and LNCaP (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0041) at 1:1000 dilution.Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (clone M004). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Integrin β1 (Extracellular region) Antibody
$379
Immunocytochemical labeling of Hsp60 in mitochondria in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal Hsp60 (HM4381). The antibody was detected using goat anti-mouse DyLight® 488.Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).
PhosphoSolutions Anti-Hsp60 (N-terminal region) Antibody
$379
Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3β (lanes 1 & 2) or anti-GSK-3α/β (Y279/Y216) (lanes 3 & 4).
PhosphoSolutions Anti-GSK-3α/β (Tyr-279/Tyr-216), Phosphospecific Antibody
$348

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