Monoclonal Antibodies

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Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MCF7 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M591). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Hyaluron Binding Region) Antibody
$370
Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M588). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Hyaluron Binding Region) Antibody
$370
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M024). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD44 (CM0241) for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$370
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$370
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M007). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$370
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).
PhosphoSolutions Anti-c-Cbl (Tyr-700), Phosphospecific Antibody
$340
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).
PhosphoSolutions Anti-c-Cbl (C-terminal region) Antibody
$285
Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody
$370
Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).
PhosphoSolutions Anti-Caveolin-1 Antibody
$370
Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody
$370
Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-δ1-Catenin (Thr-916) (lanes 3 & 4).Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-δ1-Catenin (central region) Antibody
$370
Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-β-Catenin (CM1181) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-β-Catenin Antibody
$370
Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).
PhosphoSolutions Anti-Caspase-8 (C-terminal region) Antibody
$285
Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).
PhosphoSolutions Anti-Caspase-6 (C-terminal region) Antibody
$285
Western blot analysis of Caspase-3 in multiple human tissues using CM4911 at 1:100. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in human, mouse and rat heart lysate, respectively.Induction of apoptosis after combination treatment was marked by poly (ADP-ribose) polymerase (PARP) cleavage and caspase-3 (Cat. CM4911) activation. Western blots of single or combination treatment of LNCaP (48 h), DU145, PC-3 or CHO cells (all 72 h) with 1.0 µg/ml SO1861 and 2.5 nM EGF-PE24mutΔREDLK. β-actin was used as a loading control. *CHO cells are not of human origin - detection of human Caspase-3 was not possible in this cell line. Image from publication CC-BY-4.0. PMID: 37859824
PhosphoSolutions Anti-Caspase-3 (p17 subunit) Antibody
$285
Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).Inhibition of protein biosynthesis as shown by puromycin Western blot analysis and induction of apoptosis in LNCaP cells as demonstrated by PARP and Caspase-3 (Cat. CM3771) cleavage after combination treatment. Image from publication CC-BY-4.0. PMID: 37484018
PhosphoSolutions Anti-Caspase-3 (N-terminal region) Antibody
$285
Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Calnexin (CM4371), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).
PhosphoSolutions Anti-Calnexin (N-terminal region) Antibody
$370
Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-P-Cadherin at 1:250 (lane 1) and 1:500 (lane 2), 1:1000 (lane 3) and 1:2000 (lane 4).
PhosphoSolutions Anti-P-Cadherin (N-terminal region) Antibody
$285
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). The blots were probed with anti-N-Cadherin (Cytoplasmic) (lanes 1 & 2) and anti-N-cadherin (Tyr-820) (lanes 3-6). The latter antibody was used in the presence of no peptide (lane 4), phospho-N-cadherin (Tyr-820) peptide (lane 5), or phospho-N-cadherin (Tyr-860) peptide (lane 6).Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-860) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-N-Cadherin (Cytoplasmic) Antibody
$370
Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).
PhosphoSolutions Anti-E-Cadherin (C-terminal fragment) Antibody
$285
Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-E-Cadherin (Cytoplasmic) Antibody
$370
Western blot of human LNCaP prostate adenocarcinoma (lane 1), MCF-7 breast ductal carcinoma (lane 2), NCI-H28 pleural mesothelioma (lane 3), A431 epidermoid carcinoma (lane 4), MDAMB-231 breast carcinoma (lane 5) and Jurkat T-cell line (lane 6). The blot was probed with mouse monoclonal anti-B7-H3/CD276 (BM0451) at 1:500.Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-B7-H3/CD276 (Extracellular region) Antibody
$370
Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Axl/UFO (Extracellular region) Antibody
$370
Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).
PhosphoSolutions Anti-Atrogin-1 Antibody
$340

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