Monoclonal Antibodies

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Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0331). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD55 (CM0331) for ELISA capture of human recombinant CD55 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
PhosphoSolutions Anti-CD55 (Extracellular region) Antibody
$370
Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0021). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD55 (Extracellular region) Antibody
$340
Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (CM0371). The antibody was detected using goat anti-mouse Ig:DyLight® 594.
PhosphoSolutions Anti-CD46 (Extracellular region) Antibody
$370
Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MCF7 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M591). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Hyaluron Binding Region) Antibody
$370
Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M588). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Hyaluron Binding Region) Antibody
$370
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M024). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD44 (CM0241) for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$370
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$370
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M007). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$370
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).
PhosphoSolutions Anti-c-Cbl (Tyr-700), Phosphospecific Antibody
$340
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).
PhosphoSolutions Anti-c-Cbl (C-terminal region) Antibody
$285
Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody
$370
Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).
PhosphoSolutions Anti-Caveolin-1 Antibody
$370
Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody
$370
Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-δ1-Catenin (Thr-916) (lanes 3 & 4).Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-δ1-Catenin (central region) Antibody
$370
Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-β-Catenin (CM1181) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-β-Catenin Antibody
$370
Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).
PhosphoSolutions Anti-Caspase-8 (C-terminal region) Antibody
$285
Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).
PhosphoSolutions Anti-Caspase-6 (C-terminal region) Antibody
$285
Western blot analysis of Caspase-3 in multiple human tissues using CM4911 at 1:100. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in human, mouse and rat heart lysate, respectively.Induction of apoptosis after combination treatment was marked by poly (ADP-ribose) polymerase (PARP) cleavage and caspase-3 (Cat. CM4911) activation. Western blots of single or combination treatment of LNCaP (48 h), DU145, PC-3 or CHO cells (all 72 h) with 1.0 µg/ml SO1861 and 2.5 nM EGF-PE24mutΔREDLK. β-actin was used as a loading control. *CHO cells are not of human origin - detection of human Caspase-3 was not possible in this cell line. Image from publication CC-BY-4.0. PMID: 37859824
PhosphoSolutions Anti-Caspase-3 (p17 subunit) Antibody
$285
Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).Inhibition of protein biosynthesis as shown by puromycin Western blot analysis and induction of apoptosis in LNCaP cells as demonstrated by PARP and Caspase-3 (Cat. CM3771) cleavage after combination treatment. Image from publication CC-BY-4.0. PMID: 37484018
PhosphoSolutions Anti-Caspase-3 (N-terminal region) Antibody
$285
Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Calnexin (CM4371), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).
PhosphoSolutions Anti-Calnexin (N-terminal region) Antibody
$370
Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-P-Cadherin at 1:250 (lane 1) and 1:500 (lane 2), 1:1000 (lane 3) and 1:2000 (lane 4).
PhosphoSolutions Anti-P-Cadherin (N-terminal region) Antibody
$285
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). The blots were probed with anti-N-Cadherin (Cytoplasmic) (lanes 1 & 2) and anti-N-cadherin (Tyr-820) (lanes 3-6). The latter antibody was used in the presence of no peptide (lane 4), phospho-N-cadherin (Tyr-820) peptide (lane 5), or phospho-N-cadherin (Tyr-860) peptide (lane 6).Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-860) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-N-Cadherin (Cytoplasmic) Antibody
$370
Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).
PhosphoSolutions Anti-E-Cadherin (C-terminal fragment) Antibody
$285
Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-E-Cadherin (Cytoplasmic) Antibody
$370

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