Monoclonal Antibodies

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.Immunostaining of HeLa cells showing strong mitochondrial labeling of HSP60 (Cat. 750-HSP60, 1:5000, red) and nuclear staining with DAPI (blue).
PhosphoSolutions Anti-Hsp60 Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX1 protein.Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of FOX1 (Cat. 581-FOX1, 1:500, green).
PhosphoSolutions Anti-FOX1 Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.Immunofluorescence of HeLa cells showing clear localized labeling of the DNA in the nucleus with Anti-EWS (Cat. 540-EWS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:5000, red) labels the cytoplasmic intermediate filament, and Hoechst DNA stain (blue).
PhosphoSolutions Anti-EWS Antibody
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Immunofluorescence of rat cortical neuron-glial cells showing strong cytoplasmic labeling of a small population of developing neurons and their processes with Anti-DCX (Cat. 451-DCX, 1:1000, red) while Anti-MAP2 (Cat. 1100-MAP2, 1:10,000, green) labels dendrites and perikarya or mature neurons, and additional nuclear staining was done with DAPI (blue). Anti-doublecortin is an excellent marker of early developing neuronal cells.Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 kDa and ~45 kDa doublecortin protein.
PhosphoSolutions Anti-Doublecortin Antibody
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.
PhosphoSolutions Anti-COBRA1 (NELF-B) Antibody
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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa CD75 protein.
PhosphoSolutions Anti-CD75 (Transferrin Receptor) Antibody
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Western blot of rat striatal lysate showing specific immunolabeling of the ~28 kDa Cdk5 protein.
PhosphoSolutions Anti-CDK5 Antibody
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Western blot of UV treated HeLa cell lysate showing specific immunolabeling of the ~98 kDa CD71 protein.
PhosphoSolutions Anti-CD71 Antibody
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Immunofluorescence of a section of rat cerebellum labeled with anti-calbindin (Cat. 302-CALB, 1:2,000, red), colabeled with anti-GFAP (Cat. 620-GFAP , 1:5000,green), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.
PhosphoSolutions Anti-Calbindin Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (Cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (Cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
PhosphoSolutions Anti-Vimentin Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
PhosphoSolutions Anti-Vimentin Antibody
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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (Cat. 2060-UCHL1, 1:500, red) and anti-Neurofilament H (Cat. 1451-NFH, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
PhosphoSolutions Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.
PhosphoSolutions Anti-Syntaxin Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145 kDa synaptojanin I protein.
PhosphoSolutions Anti-Synaptojanin 1 Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein. Western blot showing specific immunolabeling of Synaptobrevin (VAMP, cat. 1933-SYB, 1:1000) in mouse brain, rat brain, and lamprey spinal cord. Image kindly provided by Karina Vargas, University of Pittsburgh.
PhosphoSolutions Anti-Synaptobrevin (VAMP) Antibody
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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.
PhosphoSolutions Anti-Survival Motor Neuron (SMN) Antibody
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Immunoflourescence of HeLa cells labeled with anti-SAP49 (Cat. 1900-SAP, red, 1:1000) and anti-vimentin (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.
PhosphoSolutions Anti-SAP49 Antibody
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Immunostaining of rat dentate gyrus showing specific immunolabeling of the Prox1 protein(DAB, 1:1000). Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.
PhosphoSolutions Anti-Prox1 Antibody
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Western blot of rat kidney lysate showing specific immunolabeling of the ~21 kDa Park7 protein.Immunoflourescence of HeLa cells stained with Anti-Park7 (Cat. 1597-PARK, green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin (Cat. 2105-VIM, red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.
PhosphoSolutions Anti-Park7 (DJ-1) Antibody
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Immunostaining of HeLa cells showing specific labeling of nuclei using the anti-nuclei antibody (Cat. 1590-NUC, 1:100, green).
PhosphoSolutions Anti-Nuclei Antibody
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Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (Cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (Cat. 2105-VIM, red, 1:500).Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody (Cat. 1515-NPC, red, 1:100) and rabbit anti-vimentin antibody (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.
PhosphoSolutions Anti-Nuclear Pore Complex Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (Cat. 1454-NFM, 1:5000, red) and anti-Neurofilament-H (Cat. 1451-NFH, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
PhosphoSolutions Anti-Neurofilament NF-M Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody (Cat. 1452-NFL, green, 1:100) and anti-alpha internexin.
PhosphoSolutions Anti-Neurofilament NF-L Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.Immunostaining of human cerebellar cortex showing labeling of NF-H (Cat. 1450-NFH, DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.
PhosphoSolutions Anti-Neurofilament NF-H Antibody
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