Monoclonal Antibodies

2392 products

Monoclonal antibodies are generated from hybridoma clones and are powerful research tools for analyzing proteins to understand biological and cellular functions.

We have a broad portfolio of monoclonal antibodies, including the NeuroMab line, which was developed in collaboration with the UC Davis – NIH facility. These highly-cited, highly-validated antibodies have been trusted by the neuroscience research community for years. Knockout validation guarantees target specificity and production from hybridoma clones provides batch-to-batch consistency and long-term supply.

Analysis of multiple proteins within the same tissue sample, using immunofluorescence is key to advancing research. To support the multiplexing field we provide fluorescent conjugates which include 490, 550, 594 and 650. Our mouse monoclonal conjugates continue to grow in popularity.

Showing 481 - 504 of 2392 products

Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).

PhosphoSolutions Anti-CRMP2 (Thr-555), Phosphospecific Antibody

$340

Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk II (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

PhosphoSolutions Anti-Crk II (C-terminal region) Antibody

$285

Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).

Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-CREB (Ser-133), Phosphospecific Antibody

$285

Western blot analysis of connexin-43 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-connexin-43 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

PhosphoSolutions Anti-Connexin-43 (C-terminal region) Antibody

$285

Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals CM5051 anti-CLASP2 (clone KT68) (lanes 1 & 2) and CM5071 anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.

Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (left) and rat monoclonal anti-CLASP2 (CM5051) (middle). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).

PhosphoSolutions Anti-CLASP2 (N-terminus) Antibody

$285

Western blot of CLASP1 in mouse brain (lane 1) and rat PC12 cells (lane 2). The blots were probed with rat monoclonal CM5011 anti-CLASP1 (C-terminus) at a dilution of 1:500. Then detected using donkey anti-Rat IgG:HRP (RS3101).

PhosphoSolutions Anti-CLASP1 (C-terminus) Antibody

$285

Western blot analysis of human HeLa cells. The blot was probed with anti-Cdk5 (CM2361) antibody at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000.(lane 3).

PhosphoSolutions Anti-Cdk5 Antibody

$285

Western blot analysis of human SYF fibroblasts before (lanes 1 & 3) and after (lanes 2 & 4) treatment with alkaline phosphatase. The blots were probed with anti-Cdk1 (N-terminal region) antibody (lanes 1 & 2) or anti-Cdk1 (Tyr-15) phospho-specific antibody (lanes 3 & 4).

PhosphoSolutions Anti-Cdk1 (Tyr-15) [conserved site], Phosphospecific Antibody

$340

PhosphoSolutions Anti-Cdk1 (N-terminal region) Antibody

$285

Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-Cdc42 Antibody

$285

Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).

Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (CM1521) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.

PhosphoSolutions Anti-Cdc42 Antibody

$285

Western blot of native lysates including human CD73 recombinant protein (lane 1), NCI-H1915 lung carcinoma (lane 2), MeWo melanoma (lane 3), NCI-H1299 lung carcinoma (lane 4), and MDA-MB-231 breast carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD73 (CM0641) at 1:500.

PhosphoSolutions Anti-CD73/NT5E Antibody

$340

Native western blot of human BEAS-2B lung cells (lane 1), NCI-H446 lung cancer cells (lane 2), lung mesothelioma cells: NCI-H28 (lane 3), and NCI-H2052 (lane 4). The blot was probed with mouse monoclonal anti-CD47 (CM0541) at 1:500.

Immunocytochemical labeling of CD47 in aldehyde fixed human MCF7 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-CD47 (CM0541). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-CD47 (Extracellular region) Antibody

$370

Western blot of native (lane 1 and 3) and denatured (lane 2 and 4) lysates of human THP-1 monocytes. The blot was probed with mouse monoclonals anti-CD244/2B4/SLAMF4 (CM0531) (lanes 1 and 2) or anti-CD244/2B4/SLAMF4 (CM0851). Both antibodies were used at 1:250.

Immunocytochemical labeling of CD244 in aldehyde fixed and NP-40 permeabilized PMA-differentiated human THP-1 cells. The cells were labeled with mouse monoclonal anti-CD244/2B4/SLAMF4 (CM0851). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-CD244/2B4/SLAMF4 (Extracellular region) M085 Antibody

$370

PhosphoSolutions Anti-CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody

$370

Western blot of human A549 lung carcinoma (lane 1), NCI-H446 small cell lung carcinoma (lane 2), NCI-H1299 lung carcinoma (lane 3), MDA-MB-231 breast carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD155 (CM0481) at 1:500.

Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-CD155/PVR (Extracellular region) Antibody

$370

Western blot of lysates from SK-MES-1 squamous carcinoma (lane 1), MDA-MB-231 breast carcinoma (lane 2), MeWo melanoma (lane 3), A431 skin adenocarcinoma (lane 4), LNCaP prostate cancer cells (lane 5), MCF7 breast cancer cells (lane 6) and NCI-H1915
lung cancer cells. The blot was probed with
mouse monoclonal anti-CD147 (CM0521) at 1:1000.

PhosphoSolutions Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody

$340

Western blot of lysates from BEAS normal lung cells (lane 1), NCIH28 mesothelial cells (lane 2), NCI-H1299 lung cancer cells (lane 3), LNCaP prostate cancer cells (lane 4), MCF7 breast cancer cells (lane 5), and A431 skin adenocarcinoma (lane 6). The blot was probed with mouse monoclonal anti-CD147 (CM0361) at 1:1000.

Immunocytochemical labeling of CD147 in paraformaldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD147 (CM0361). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

PhosphoSolutions Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody

$370

Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M030). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-CD99 (Extracellular region) Antibody

$340

Western blot analysis of human cell lysates: A549 (lane 1), A431 (lane 2), LNCaP (lane 3), MDA-MB-231 (lane 4) and MeWo (lane 5). The blot was probed with mouse monoclonal anti-CD99 (CM0261) at 1:1000.

PhosphoSolutions Anti-CD99 (Extracellular region) Antibody

$370

Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight® 594.

PhosphoSolutions Anti-CD63 (Extracellular region) Antibody

$340

Representative Standard Curve using mouse monoclonal anti-CD63 (CM0341) for ELISA capture of human recombinant CD63 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

PhosphoSolutions Anti-CD63 (Extracellular region) Antibody

$370

Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).

Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M015). The antibody was detected using goat anti-mouse Ig DyLight® 594.

PhosphoSolutions Anti-CD59 (glycoprotein) Antibody

$370

PhosphoSolutions Anti-CD59 (glycoprotein) Antibody

$370

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