Anti-Nestin Antibody (4D11)

Name: Anti-Nestin Antibody
Brand: PhosphoSolutions
SKU: 1435-NES
Price: 456.0 USD
Availability: InStock

Our Anti-Nestin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Nestin and is Protein G purified. It is great for use in WB, IHC, ICC.

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.

Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (Cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (Cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (Cat. 1435-NES, red, 1:500) and anti-vimentin antibody (Cat. 2105-VIM, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

Immunolabeling of the coronal section of an embryonic day 15.5 mouse cortex showing specific labeling of Nestin (Cat. 1435-NES, 1:500; green). Nuclei stained with DAPI (blue). Image kindly provided by Alyssa Treptow, University of California, Riverside.

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1435-NES

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Nestin

Scientific Background

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.

Format

Protein G Purified

Clonality

Monoclonal

Clone

4D11

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

NES

Molecular Weight

220-240 kDa

Immunogen

Recombinant human Nestin expressed in and purifeid from E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492158

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:2000
WB Brain: 1:2000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~220-240 kDa nestin doublet.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin

UniProt ID

P48681
UniProt Summary: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). {ECO:0000250}.

NCBI Gene ID

10763

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Anti-Nestin Antibody (4D11)

SKU: 1435-NES

Product Details

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