Anti-α-Tubulin (C-terminus) Antibody (DM1A)

Name: Anti-α-Tubulin (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: TM4111
Price: 292.0 USD
Availability: InStock

Our α-Tubulin (C-terminus) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat α-Tubulin (C-terminus) and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.

Immunocytochemical labeling of α- and βI-Tubulin in rat A7r5 cells. The cells were labeled with anti-βI-Tubulin (TM1541) (left) and anti-α-tubulin (TM4111) (right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 488.

Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: TM4111

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

α-Tubulin (C-terminus)

Scientific Background

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

Format

Protein A Purified

Clonality

Monoclonal

Clone

DM1A

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

TUBA1

Molecular Weight

Immunogen

Clone DM1A was generated from purified chick brain tubulin. The antibody recognizes an epitope within the C-terminal end of α-tubulin isoforms. The epitope is highly conserved in α-tubulin isoforms from most vertebrate species.

Immunogen Species

Chicken

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 55 kDa* protein corresponding to the molecular mass of α-Tubulin on SDS-PAGE immunoblots of human, rat, and mouse cells and tissues.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

alpha Tubulin, Tubulin A1C

UniProt ID

P02552

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Anti-α-Tubulin (C-terminus) Antibody (DM1A)

SKU: TM4111

Product Details

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