Fluoro NAD/NADH Detection Kit by Cell Technology

Name: Fluoro NAD/NADH Detection Kit by Cell Technology
Brand: ImmunoChemistry Technologies
SKU: FLNADH100-3
Price: 2924.0 USD
Availability: InStock

14 Citations

The kit uses a fluorescent readout to specifically detect NAD/NADH, with no reactivity with NADP/NADPH. It is suitable for use with cells and tissue from a range of sources such as animals, plants, bacteria and fungi.

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

Figure 3. The assay reacts with NAD and NADH. There is no reactivity with NADPH.

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Click on image to zoom

Go to Full Product Details

Data Sheet

Protocol

Safety Data Sheet

SKU: FLNADH100-3

Size: 500 Tests

Variant

Price:

$2,924.00

Quantity:

Ships: 1-2 business days

Product Details

Scientific Background

The role of pyridine nucleotides (NAD/NADH) metabolism in health is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation. These enzymes are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling, which suggests that either the enzymes or NAD could be an important therapeutical target.

The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.

The kit is suitable for applications such as:
- Detection of NAD/NADH activity in cells or tissue extracts.
- Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.
- NAD/NADH detection in bacterial, fungal and plant cells.

Excitation / Emission

530-570nm/590-600nm

Method of Analysis

Fluorescence plate reader

Usage Statement

For research use only. Not for use in diagnostic procedures.

Country of Origin

United States

Storage

Store contents as labeled

Shipping

Ships overnight (domestic), International Priority Shipping

FLNADH100-2: 100 Tests

Part# 6021: Enzyme Mix x 1 (-20C)

Part# 4018: NADH Detection Reagent x 1 (-20C)

Part# 7013: NADH Standard x 1 (-20C)

Part# 3044: NAD/NADH Reaction Buffer x 1 (2-8 C)

Part# 3045: NAD/NADH Lysis Buffer x 1 (2-8 C)

Part# 3046: NAD Extraction Buffer x 1 (2-8 C)

Part# 3047: NADH Extraction Buffer x 1 (2-8 C)

Part# 3051: NADH Standard Diluent x 1 (2-8 C)

FLNADH100-3: 500 Tests

Part# 6021: Enzyme Mix x 5 (-20C)

Part# 4018: NADH Detection Reagent x 5 (-20C)

Part# 7013: NADH Standard x 5 (-20C)

Part# 3044: NAD/NADH Reaction Buffer x 5 (2-8 C)

Part# 3045: NAD/NADH Lysis Buffer x 5 (2-8 C)

Part# 3046: NAD Extraction Buffer x 5 (2-8 C)

Part# 3047: NADH Extraction Buffer x 5 (2-8 C)

Part# 3051: NADH Standard Diluent x 5 (2-8 C)

Product Specific References

PMID	Publication
35504207	Luan, Y., et al. 2022. STING modulates necrotic cell death in CD4 T cells via activation of PARP-1/PAR following acute systemic inflammation. nt Immunopharmacol, 108809.
33593944	Won, H.I., et al. 2021. Inactivation of the Pta-AckA Pathway Impairs Fitness of Bacillus anthracis during Overflow Metabolism. J Bacteriol, e00660-20.
28299860	Sadykov, M.R., et al. 2017. Poly(3-hydroxybutyrate) fuels the tricarboxylic acid cycle and de novo lipid biosynthesis during Bacillus anthracis sporulation . Mol Microbiol, 793-803.
27374086	Yin, C., et al. 2016. Exogenous pyruvate facilitates cancer cell adaptation to hypoxia by serving as an oxygen surrogate. Oncotarget, 47494-47510.
27340697	Gries, C.M., et al. 2016. Potassium Uptake Modulates Staphylococcus aureus Metabolism. mSphere, e00125-16.
26322513	Esteve-Gassent, M. D., et al. 2015. Absence of sodA Increases the Levels of Oxidation of Key Metabolic Determinants of Borrelia burgdorferi. PLoS One, e0136707.
24670634	Birsoy, K., et al. 2014. Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides. Nature, 108-12.
23625849	Sadykov, M.R., et al. 2013. Inactivation of the Pta-AckA pathway causes cell death in Staphylococcus aureus. J Bacteriol, 3035-44.
23703906	Wiley, S.E., et al. 2013. Wolfram Syndrome protein, Miner1, regulates sulphydryl redox status, the unfolded protein response, and Ca2+ homeostasis. EMBO Mol Med, 904-18.
23703322	Montero, J., et al. 2013. p53 regulates a non-apoptotic death induced by ROS. Cell Death Differ, 1465-74.
23911327	De Bock, K., et al. 2013. Role of PFKFB3-driven glycolysis in vessel sprouting . Cell, 651-63.
22305999	Martin, J. A., et al. 2012. Mitochondrial electron transport and glycolysis are coupled in articular cartilage. Osteoarthritis Cartilage, 323-9.
22384140	Schaue, D., et al. 2012. Cellular autofluorescence following ionizing radiation. PLoS One, e32062.
19217932	Bickler, P.E., et al. 2009. Inositol 1,4,5-triphosphate receptors and NAD(P)H mediate Ca2+ signaling required for hypoxic preconditioning of hippocampal neurons. Neuroscience, 51-60.

Recover password

Create my account

Fluoro NAD/NADH Detection Kit by Cell Technology

SKU: FLNADH100-3

Product Details

Product Specific References

Bulk Order Fluoro NAD/NADH Detection Kit by Cell Technology

Related Products

Recently Viewed