PrecipHen® Immunoprecipitation Reagent
Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with chicken antibodies in immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and protein purification applications.
Western blot of HIF1α immunoprecipitated, using Aves Lab's PrecipHen® (Cat. P-1010), from brain lysates of fish exposed for 6 h to normoxia (>7 mg O2 l−1; lanes 2, 5, 8, 10, and 11) or hypoxia (∼1 mg O2 l−1; lanes 3-4, 6-7, and 9). A positive HIF1α control is shown in lane 1 and the mobility of HIF1α and chicken IgY are shown by arrows (at left). HIF1α protein abundance in each sample was expressed as the ratio of the HIF1α band intensity to the IgY band intensity. Image CC-BY-4.0. PMID:38116983
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SKU: P-1010
Product Details
Chicken IgY
IgG
Store at 4°C in the dark. Under these conditions, PrecipHen® has a shelf life of at least 12 months (provided it remains sterile).
Agarose beads in buffer
HEPES (50 mM, pH 7.6), EDTA (5 mM), sodium azide (0.02%, w/v) (as an anti-microbial agent).
The capacity of PrecipHen® was determined by measuring the 2.5 mLs of PrecipHen. One milliliter aliquots of chicken IgY (200 µg/mL in TE buffer) were applied to the column and fractions were collected. A similar column of agarose (non-NHS activated) was used for comprison. The concentration of IgY passing through each column was measured, and the total amount of IgY that bound to the column was calculated.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
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