ImmunoChemistry Technologies

302 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 25 - 48 of 302 products

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Mammalian, 1X

From $71.85

Figure 1. Stabilization of HRP conjugates

Figure 2. Minimizes background in mono-poly sandwich ELISAs

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Non-Mammalian-based, 1X

From $71.55

ImmunoChemistry Technologies Plasma Sample Diluent

From $60.30

Figure 1. MR-(DEVD)2 (Cat. 936) and Hoechst 33342 were used to quantify apoptosis in human PASMCs. Cells were treated with a negative control (A), a drug that inhibits proliferation (B), or H2O2 to induce apoptosis (C). Apoptotic cells stained red with cleaved MR-(DEVD)2 and blue with Hoechst. They have less intense blue nuclei than healthy cells, which have bright blue nuclei. Research of Dr. F. Perros, et al., Universite Paris-Sud 11; Clamart; Hopital Henri-Mondor, Creteil, France.

Figure 2. THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

ImmunoChemistry Technologies Hoechst 33342 Fluorescent Nucleic Acid Stain

$23.90

Figure 1. R110-(RR)2 was reconstituted in DMSO, diluted in an aqueous buffer, and then spiked with cathepsin B enzyme and incubated at 37°C for 1 hour. After incubation, fluorescence was analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.

Figure 2. Jurkat cells were treated with the cathepsin inhibitors CA-074 Me (blue) and E-64d (gray), or were untreated (green) and then stained with R110-(RR)2. Samples were then analyzed analyzed by flow cytometry (488 nm excitation and a 530/30 emission filter). Treatment with CA-074 Me or E-64d decreased the fluorescence signal detected compared to the untreated control, which displayed a baseline level of cathepsin activity. Data courtesy of Dr. Kristi Strandberg (ICT 231:40-42).

ImmunoChemistry Technologies Green Fluorescent Cathepsin B Assay

From $217.70

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin L Assay Kit

From $216.90

Figure 2. MCF-7 cells were exposed to 0.15 µM camptothecin (Cat. 6210) for 24 hours at 37°C, then stained with MR-(DEVD)2 for 30 minutes at 37°C, washed, and then stained with 1 µg/mL Hoechst stain. Apoptotic cells with orange-red lysosomal bodies and less intense blue nuclei are intermixed with non-apoptotic cells bearing bright blue nuclei and absent or reduced orange-red lysosomal staining. Photo provided by Dr. Zbigniew Darzynkiewicz at Brander Cancer Research Center Institute, New York, NY.

ImmunoChemistry Technologies Magic Red® Fluorescent Caspase-3/7 Assay Kit

From $210.20

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

ImmunoChemistry Technologies Fluoro NAD/NADH Detection Kit by Cell Technology

From $622.45

ImmunoChemistry Technologies Assay Diluent Optimization Pack

$204.70

Figure 1. Using FAM-FLICA®, non-apoptotic cells (unstained, left of histogram) can easily be distinguished from apoptotic cells exhibiting green fluorescence (right of histogram). Jurkat cells were either treated with DMSO as control (A), or 1 µM staurosporine (B) to induce apoptosis. Cells were stained with FAM-FLICA® and analyzed using a flow cytometer in FL-1. Only 7% of non-induced cells (A, right) are apoptotic compared with 96.5% of the induced cells (B, right). (ICT 226:17-19.)

Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were either untreated (A), or treated with 90% ethanol for 60 seconds, then stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.

ImmunoChemistry Technologies Necrosis vs Apoptosis Assay Kit

From $358.70

ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Test

From $182.10

ImmunoChemistry Technologies 96-Well Plate Template Post-It Notes

$8.15

Figure 1. Dilute samples within the detection limits of the ELISA

ImmunoChemistry Technologies Sample Diluent - Non-Mammalian-based

From $60.50

ImmunoChemistry Technologies Assay Diluent - Non-Mammalian

From $60.30

ImmunoChemistry Technologies Costar® 96-Well EIA/RIA Stripwell™ Plate

$7.65

Figure 1. Monocytes can secrete active caspase-1, therefore cells were labeled with FAM-FLICA® Caspase-1 (WEHD) (Cat. 9161/9162) prior to treatment. Cells were then treated with DMSO (control) or 10 µM nigericin (Cat. 6698), stained with Hoechst 33342, fixed (Cat. 636), and viewed using EGFP (Ex 470/30, Em 530/50) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Increased levels of FAM-FLICA® staining were seen in the nigericin-treated cells. Data courtesy of Dr. Kristi Strandberg, ICT (235:60).

ImmunoChemistry Technologies Green Fluorescent FAM FLICA® Caspase-1 (WEHD) Assay Kit

From $247.35

Figure 1. The process of autophagy is an intracellular degradation process during which cytosolic organelles and materials are enclosed within an isolation membrane to form an autophagosome. The outer membrane of the autophagosome fuses with the lysosome. The sequestered material is subsequently degraded within the autolysosome.

Figure 2. Cells were either untreated (Black) or treated with 0.5 µM Rapamycin (Orange), 10 µM Chloroquine (Blue), or both 0.5 µM Rapamycin and 10 µM Chloroquine (Red) for 18 hours. After staining with Autophagy Probe - Red Fluorescent, cells were washed and analyzed by flow cytometry (561 nm excitation and a 610/20 emission filter). Treatment with rapamycin or chloroquine increased the fluorescence signal compared to the control. Data courtesy of Dr. Kristi Strandberg (ICT 228:37-40).

ImmunoChemistry Technologies Autophagy Assay - Red Fluorescent

From $210.50

Figure 1. Cell death in primary rat hippocampal neurons. (A) is a composite of FLICA® FAM-DEVD-FMK (B), PI (C), and Hoechst (D). DNA is labeled in 4 cells (D), 3 cells are caspase-positive (B), 2 are membrane-compromised (C). One cell is in early apoptosis (green but not red). Two FLICA®-positive cells are also PI-positive (red, C) (becoming membrane compromised) and are in the late stages of apoptosis rather than necrosis. Data courtesy of Dr. Z. Kahraman Akozer, University of Maryland.

Figure 2. Normal (A) and keratoconus (B) corneal fibroblasts were treated with 200 μM H2O2 for 1 hour, washed, and allowed to recover. Cells were then treated with FAM-DEVD-FMK (Cat. 93/94) for 1 hour, then washed with 1X Apoptosis Wash Buffer. Keratoconus corneal fibroblasts treated with H2O2 (B) show a significant increase in caspase-3/7 activity compared to normal cells (A). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, University of California, Irvine.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $227.60

ImmunoChemistry Technologies Donkey Serum

$71.55

ImmunoChemistry Technologies Protein G Conjugated with FITC

$191.15

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Goat Serum

From $61.30

ImmunoChemistry Technologies Streptavidin/Biotin Blocking Kit set

From $78.70

ImmunoChemistry Technologies Immuno Fluoro Mount with DABCO™

From $84.80

The cell morphology of MDA-MB-231 breast cancer cells after they were treated with fludioxonil(Flu) for 72 h and were stained with DAPI and actin. The samples were sealed with mounting medium (Cat. AR-6515). Image from publication, CC-BY-4.0, PMID: 39201710.

ImmunoChemistry Technologies Fluoroshield™ with DABCO

From $89.95

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