Pepsin Reagent (Ready to Use)
Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Pepsin Reagent (Ready to Use)
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Ships: 1-2 business days
- Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer.
- Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section.
- Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.).
- Discard Pepsin reagent, wash 3-5 times with buffer.
- The tissue sections are ready for further IHC protocol.
Product Specific References
| PMID | Publication |
| 39787707 | Millares-Ramirez, EM, et al. 2025. Bronchial vessel density is correlated with airway smooth muscle cell proliferation in horses with mild and moderate asthma. American Journal of Veterinary Research, 45663. |
| 34048095 | Millares-Ramirez, EM, et al. 2021. Bronchial angiogenesis in horses with severe asthma and its response to corticosteroids. Journal of Veterinary Internal Medicine, 2026-2034. |