Fluorescent ADP Detection Kit
The kit utilizes a non-fluorescent detection reagent, which is reduced in the presence ADP and a coupled enzyme reaction to produce its fluorescent analog. The kit can be used with mammalian cells and tissues, and bacterial, fungal and plant cells.
Figure 1. Assay principle
Click on image to zoom
Ships: 1-2 business days
FLADP100-2: 100 Tests
1. Part #: 6031. Enzyme Mix 25X: 1 frozen vial 220 μL. Upon arrival store at -20 °C.
Aliquot and store at -20 °C.
2. Part# 3064: Substrate Buffer. 1 bottle 25mL. Upon arrival store at -20 °C. Ready to use.
3. Part# 4028: Detection Reagent 100X: 1 dried vial. Upon arrival store -20 °C
To reconstitute add 60 μL of anhydrous DMSO. Aliquot and store at -20 °C
4. Part# 7025: ADP Standard 2.5mM: 1 vial. Upon arrival store -20 °C
FLADP100-3: 500 Tests1. Part #: 6031. Enzyme Mix 25X: 5 frozen vials 220 μL. Upon arrival store at -20 °C.
Aliquot and store at -20 °C.
2. Part# 3064: Substrate Buffer. 5 bottle 25mL. Upon arrival store at -20 °C. Ready to use.
3. Part# 4028: Detection Reagent 100X: 5 dried vials. Upon arrival store -20 °C
To reconstitute add 60 μL of anhydrous DMSO. Aliquot and store at -20 °C
4. Part# 7025: ADP Standard 2.5mM: 5 vials. Upon arrival store -20 °C
After first use aliquot and store at -20 °C
5. Part# 7026: NEM: 5 vials dried. Reconstitute in 0.5mL of ETOH. Upon arrival and reconstitution store -20 °C. Dilute as needed After first use aliquot and store at -20 °C
5. Part# 7026: NEM: 1 vial dried. Reconstitute in 0.5mL of ETOH. Upon arrival and reconstitution store -20 °C. Dilute as needed
FLADP100-3: 500 Tests
Product Specific References
| PMID | Publication |
| 31489369 | Karch, J., et al. 2019. Inhibition of mitochondrial permeability transition by deletion of the ANT family and CypD. Sci Adv, eaaw4597. |