Streptavidin Conjugated with Peroxidase

Name: Streptavidin Conjugated with Peroxidase
Brand: ImmunoChemistry Technologies
SKU: AR-6633-01
Price: 184.0 USD
Availability: InStock

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Applications

ELISA, ICC, IHC, WB

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SKU: AR-6633-01

Volume: 1 mg

Variant

Price:

$184.00

Quantity:

Ships: 1-2 business days

Product Details

Scientific Background

Streptavidin is a 52-66 kDa (average 60 KDa) (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0 One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.

Applications

ELISA, ICC, IHC, WB

Buffer

The conjugate (one mg of streptavidin conjugated with peroxidase) is supplied in one ml of 10 mM PO4, 200 mM NaCl, pH 7.5, BSA 10 mg/ml and 0.05% Proclin 300.

Usage Statement

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Country of Origin

United States

Storage

2-8 °C

Shipping

Ships overnight (domestic), International Priority Shipping

This product can be diluted in our Immuno Automation buffer IBSC cat # AR-6561 or PBS.

BSA 0.5-1.0% should be added in these solutions.

This product can also be diluted in our Universal antibody dilution buffer (AR-6562) without adding any carrier protein.

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Streptavidin Conjugated with Peroxidase

SKU: AR-6633-01

Product Details

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