Immuno In Situ Mount™
Immuno in Situ mounting medium is a unique mounting medium designed for permanent preservation of tissue sections and cell smears with alkaline phosphatase chromogens like NBT/BCIP and Immuno Red counter stain™ (protein stain) (IBSC cat. # AR-6523).
Immuno In Situ Mount™
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Ships: 1-2 business days
When the room temperature is low, 20ºC or lower, this mounting medium will become gel. Warm mounting medium to approximately 25ºC and apply as described below.
- Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
- Turn the vial upside and remove any air bubbles.
- Apply 3-4 drops directly on the top of specimen and spread out evenly by tilting back and forth or spread evenly with a 0.2 ml plastic pipette tip.
- Let stand at room temperature for about 60-90 minutes or heat slides at 37-40oC for 40-50 minutes. A COVERSLIP IS NOT REQUIRED.
- The specimen is ready for visualization under a microscope.
- To visualize slide under microscope with 4X, 10X, 20X or 40X lens coverslip is NOT required At this step for any reason if coverslip has to be removed, follow these instructions: Soak the slide in warm (37°C)distilled or deionized water for 5-10 minutes until the mounting medium is dissolved. Rinse slide with warm water several times to remove all mounting medium; the slide can be remounted again if required.
For Oil Immersion lens, the slide can be visualized as follows: A drop of oil can be placed directly on dried mounting medium for visualization; the oil can be removed by wiping with Kimwipes or lens paper.
OR Apply 1-2 drops of any organic mounting medium on top of dried mounting medium. which has protected the specimen, cover slide with coverslip; the slide is ready for visualization.