Anti-Epithelial Membrane Antigen (EMA) (EMA 201)

Name: Anti-Epithelial Membrane Antigen (EMA)
Brand: Antibodies Incorporated
SKU: MM-1001
Price: 337.0 USD
Availability: InStock

Our mouse Epithelial Membrane Antigen (EMA) monoclonal antibody from Antibodies Incorporated is produced in-house from clone EMA 201. It detects human Epithelial Membrane Antigen (EMA) and is purified by Protein A chromatography. Works in IHC, ICC, WB, ELISA.

Immunofluorescent staining of MCF7 cells 5 ug/mL mouse monoclonal anti-Epithelial Membrane Antigen (EMA) antibody (MM-1001) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-EMA stains the membrane of MCF7 cells.

Western blotting of MCF7 cell lysate using 1 ug/mL and 5 ug/mL mouse monoclonal anti-Epithelial Membrane Antigen (EMA) antibody (MM-1001). MM-1001 mouse anti-Epithelial Membrane Antigen antibody recognizes endogenous EMA in the range of 260-400 kDa.

Immunofluorescent staining of MCF7 cells 5 ug/mL mouse monoclonal anti-Epithelial Membrane Antigen (EMA) antibody (MM-1001) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-EMA stains the membrane of MCF7 cells.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: MM-1001

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Epithelial Membrane Antigen (EMA)

Scientific Background

Epithelial membrane antigens are high molecular weight mucin-like glycoproteins. Antibodies to EMA have been shown to be useful as pan-epithelial markers for detection of early metastatic loci of carcinoma in the bone marrow or liver.

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

EMA 201

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

MUC1

Molecular Weight

260-400 kDa

Immunogen

Human milk fat globule membranes

Immunogen Species

Human

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ADMCKD; CD227; CA 15-3; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM; mucin-1; H23 antigen; breast carcinoma-associated antigen DF3; cancer antigen 15-3; carcinoma-associated mucin; episialin; krebs von den Lungen-6; mucin 1; transmembrane; peanut-reactive urinary mucin; polymorphic epithelial mucin; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen; MUC1; CA15-3

UniProt ID

P15941
UniProt Summary: The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.; The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

NCBI Gene ID

4582

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Epithelial Membrane Antigen (EMA) (EMA 201)

SKU: MM-1001

Product Details

Bulk Order Anti-Epithelial Membrane Antigen (EMA)

Related Products

Recently Viewed