Rat Multi-Neurotrophin Screening Rapid ELISA Assay

Name: Rat Multi-Neurotrophin Screening Rapid ELISA Assay
Brand: Biosensis
SKU: BEK-2232-SET
Price: 617.0 USD
Availability: InStock

The Biosensis Multi-Neurotrophin Rapid Screening ELISA kit has been designed to allow rapid screening and quantification of rat NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum and brain extracts. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid Screening ELISA kit presents a cost-effective way to quickly screen multiple samples. It comes complete with all detection reagents and is ready to use.

These standard curves generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

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Species Reactivity

Rat

Applications

ELISA

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Data Sheet

SKU: BEK-2232-SET

Volume/Size: 4 x 6 Strips Set

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Price:

$617.00

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Product Description

The Biosensis Multi-Neurotrophin Rapid Screening ELISA kit has been designed to allow rapid screening and quantification of rat NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum.

This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded.

NOTE: For research use only. Not for diagnostic and clinical purposes.

Scientific Background

The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.

Target

Multi-Neurotrophin Screening

Immunogen

As for individual ELISA kits

Immunogen Species

Rat

Range

NGF (3.9 - 250 pg/mL) BDNF (7.8 - 500 pg/mL) NT3 (15.6 - 1,000 pg/mL) NT4/5 (15.6 - 1,000 pg/mL)

Sensitivity

Typical LODs achieved based on 150% of background OD:NGF: 3 pg/mLBDNF: 1 pg/mLNT3: 4 pg/mLNT4/5: 30 pg/mL

Specificity

This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.

Species Reactivity

Rat

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Cell Lysates, Culture Supernatant, Serum, Tissue Homogenates

Synonyms

NGF; BDNF; NT3; NT4/5

UniProt ID

P23363; P25427; P18280; P31413

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months from purchase.

Storage

Store at 2-8°C

Shipping

Shipped on ice packs

Rat Multi-Neurotrophin Screening Rapid ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

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Rat Multi-Neurotrophin Screening Rapid ELISA Assay

SKU: BEK-2232-SET

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