Human ApoE/A beta ELISA Assay
Biosensis is proud to offer the first commercially available ApoE/β-amyloid (ApoE/Aβ) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/Aβ complex in tissue extracts and other samples. The Biosensis ApoE/Aβ Complex ELISA kit is a sandwich ELISA.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
Note: while the concentration of ApoE and monomeric Aβ peptide used to generate the standard is accurately determined prior to complex formation, the precise characteristics, size and number of ApoE/Aβ complexes cannot be quantified by any known method. Consequently, results should be presented as a percentage rather than an absolute concentration. This is shown in the graph and also as published by Tai et al. (2013, 2014).
Click on image to zoom
Note: while the concentration of ApoE and monomeric Aβ peptide used to generate the standard is accurately determined prior to complex formation, the precise characteristics, size and number of ApoE/Aβ complexes cannot be quantified by any known method. Consequently, results should be presented as a percentage rather than an absolute concentration. This is shown in the graph and also as published by Tai et al. (2013, 2014).
| Reagent | 1 Plate Kit | 2 Plate Kit |
|---|---|---|
| MOAB-2 Antibody Coated 96 well microplate | 96 wells | 192 wells |
| Control Antibody Coated 96 well microplate | 96 wells | 192 wells |
| Assay Diluent D, 2X | 25 mL x 2 | 25 mL x 4 |
| Lyophilized ApoE/Aβ Complex Standard | 2 Vials | 4 Vials |
| ApoE/Aβ Complex Detection Antibody | 220 μL x 1 | 220 μL x 2 |
| Streptavidin-HRP, 100X | 220 μL x 1 | 220 μL x 2 |
| Wash Buffer, 20X | 33 mL x 1 | 33 mL x 2 |
| TMB Substrate, 1X | 22 mL x 1 | 22 mL x 2 |
| TMB Stop Solution, 1X | 22 mL x 1 | 22 mL x 2 |
| Plate sealer | Supplied | |