ELISA Kits and Reagents

ImmunoChemistry Technologies ABTS 1-Component HRP Microwell Substrate (SUBA)

$45.40

Figure 1. In Antigen-Down-ELISAs, a Target Antigen coated onto the ELISA plate surface is used to quantify specific antibody concentrations present within samples. When the sample is added, the antigen on the plate is bound by antibodies from the sample, which are then retained in the well. A species-specific antibody labeled with HRP is added, followed by substrate, a stop solution is added, and then the absorbance is read. The higher the signal, the more antibodies there are in the sample.

Figure 2. Initial Assay Feasibility Plate Map. Wells are coated with 100 µL per well of either 1 µg/mL (light gray) or 4 µg/mL (dark gray) antigen coating solution, or Antigen Coating Buffer blank well controls (white). A known Positive Control is diluted out via a series of 1:2 dilutions and added to six sections of the plate. After the antibody capture step, plates are incubated with three different dilutions of the HRP-IgG conjugate, washed, and developed with TMB substrate. See protocol.

ImmunoChemistry Technologies Antigen-Down ELISA Development Kit

$590

ImmunoChemistry Technologies Antibody-Sandwich ELISA Development Kit

$669

ImmunoChemistry Technologies General Assay Diluent, 2X

$562

ImmunoChemistry Technologies General Serum Diluent, 2X

$562

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Mammalian, 1X

From $70.30

Figure 1. HRP-Maleimide-conjugated to thiolated antibody. Our Conjugation-Ready HRP-Maleimide reagent (HRP-M) makes it easy for scientists to create their own detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl (SH) groups on the target antibody (shown) or other target protein.

Figure 2. Thiolation. Conjugation-Ready HRP Maleimide links to proteins through free SH groups, which often must be added to the protein using Traut’s reagent prior to conjugation.

ImmunoChemistry Technologies Conjugation-Ready HRP Maleimide

$227

ImmunoChemistry Technologies HRP Goat Anti-Rabbit IgG Fc

$150

ImmunoChemistry Technologies HRP Goat Anti-Mouse IgG Fc

$150

ImmunoChemistry Technologies HRP Goat Anti-Human IgG Fc

$150

ImmunoChemistry Technologies Pack of Desiccant Packets

$8

ImmunoChemistry Technologies Stop Solution for AP Substrates (STOPP)

$26

ImmunoChemistry Technologies BCIP/NBT 1-Component AP Membrane Substrate (SUBB)

$66

ImmunoChemistry Technologies TMB 1-Component HRP Membrane Substrate (SUBM)

$66

ImmunoChemistry Technologies pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)

$49

ImmunoChemistry Technologies TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)

$58.20

ImmunoChemistry Technologies Alkaline Phosphatase Conjugate Stabilizer, 1X

From $28.20

ImmunoChemistry Technologies Phosph-Free Block ELISA Blocking Buffer

From $53.80

Figure 1. Optimization of double-antigen sandwich ELISA. (a) Capture (0.5 to 5.0 µg/mL) and detection antigen (0.2 (green), 0.4 (light blue), 0.8 (yellow), and 1.6 (purple)) were used to optimize capture and detection antigen (N protein). Capture antigen was diluted with Antigen Coating Buffer. From Figure 1a of Cordero-Ortiz, M, et al. (2023) Animals (Basel) 13, 3487 doi: 10.3390/ani13223487. Used under CC License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.

ImmunoChemistry Technologies Antigen Coating Buffer, 5X

From $55.90

ImmunoChemistry Technologies Phosphate Buffered Saline (PBS), 10X

From $17.30

ImmunoChemistry Technologies Antigen-Down HRP Conjugate Stabilizer, 5X

From $70.30

ImmunoChemistry Technologies Antibody Coating Buffer, 5X

From $55.90

Figure 1. Equalize the matrix with an assay diluent

ImmunoChemistry Technologies Antigen-Down Assay Diluent

From $59

ImmunoChemistry Technologies Neptune Assay Diluent

From $59

Recover password

Create my account

ELISA Kits and Reagents

Recently Viewed