ELISA Kits & Reagents

ImmunoChemistry Technologies TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)

$59.45

ImmunoChemistry Technologies pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)

$50.10

ImmunoChemistry Technologies TMB 1-Component HRP Membrane Substrate (SUBM)

$67.45

ImmunoChemistry Technologies BCIP/NBT 1-Component AP Membrane Substrate (SUBB)

$67.45

ImmunoChemistry Technologies Stop Solution for TMB Substrates (STOPT)

From $31.45

ImmunoChemistry Technologies Stop Solution for AP Substrates (STOPP)

$26.55

ImmunoChemistry Technologies ELISA Plate Sealing Covers

$10.50

ImmunoChemistry Technologies Foil Storage Bags for ELISA Plates

$8.15

ImmunoChemistry Technologies Pack of Desiccant Packets

$8.15

ImmunoChemistry Technologies 96-Well Plate Template Post-It Notes

$8.15

ImmunoChemistry Technologies HRP Goat Anti-Human IgG Fc

$153.30

ImmunoChemistry Technologies HRP Goat Anti-Mouse IgG Fc

$153.30

ImmunoChemistry Technologies HRP Goat Anti-Rabbit IgG Fc

$153.30

Figure 1. HRP-Maleimide-conjugated to thiolated antibody. Our Conjugation-Ready HRP-Maleimide reagent (HRP-M) makes it easy for scientists to create their own detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl (SH) groups on the target antibody (shown) or other target protein.

Figure 2. Thiolation. Conjugation-Ready HRP Maleimide links to proteins through free SH groups, which often must be added to the protein using Traut’s reagent prior to conjugation.

ImmunoChemistry Technologies Conjugation-Ready HRP Maleimide

$232

ImmunoChemistry Technologies Monster Block ELISA Blocking Buffer

From $55.20

ImmunoChemistry Technologies Alternative Block ELISA Blocking Buffer

From $55.20

Figure 1. Stabilization of HRP conjugates

Figure 2. Minimizes background in mono-poly sandwich ELISAs

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Non-Mammalian-based, 1X

From $71.55

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Mammalian, 1X

From $71.85

ImmunoChemistry Technologies Protein-Free Sample Diluent

From $49.25

ImmunoChemistry Technologies General Serum Diluent, 2X

$574.40

ImmunoChemistry Technologies General Assay Diluent, 2X

$574.40

ImmunoChemistry Technologies Antibody-Sandwich ELISA Development Kit

$683.75

Figure 1. In Antigen-Down-ELISAs, a Target Antigen coated onto the ELISA plate surface is used to quantify specific antibody concentrations present within samples. When the sample is added, the antigen on the plate is bound by antibodies from the sample, which are then retained in the well. A species-specific antibody labeled with HRP is added, followed by substrate, a stop solution is added, and then the absorbance is read. The higher the signal, the more antibodies there are in the sample.

Figure 2. Initial Assay Feasibility Plate Map. Wells are coated with 100 µL per well of either 1 µg/mL (light gray) or 4 µg/mL (dark gray) antigen coating solution, or Antigen Coating Buffer blank well controls (white). A known Positive Control is diluted out via a series of 1:2 dilutions and added to six sections of the plate. After the antibody capture step, plates are incubated with three different dilutions of the HRP-IgG conjugate, washed, and developed with TMB substrate. See protocol.

ImmunoChemistry Technologies Antigen-Down ELISA Development Kit

$603

ImmunoChemistry Technologies Assay Diluent Optimization Pack

$204.70

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ELISA Kits & Reagents

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