Anti-Caspase-3 (N-terminal region) Antibody (M377)

Name: Anti-Caspase-3 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM3771
Price: 292.0 USD
Availability: InStock

Our Caspase-3 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Caspase-3 (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

Inhibition of protein biosynthesis as shown by puromycin Western blot analysis and induction of apoptosis in LNCaP cells as demonstrated by PARP and Caspase-3 (Cat. CM3771) cleavage after combination treatment. Image from publication CC-BY-4.0. PMID: 37484018

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: CM3771

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Caspase-3 (N-terminal region)

Scientific Background

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M377

Isotype

IgG2a

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

CASP3

Molecular Weight

Immunogen

Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* protein corresponding to the molecular weight of Caspase-3 on SDS-PAGE immunoblots of human Jurkat and K562 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Caspase,-3, CPP-32, Apoptain, Yama, SCA-1, CASP-2, NEDD2, ICH-1, p18, p13, p12

UniProt ID

P42574

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Anti-Caspase-3 (N-terminal region) Antibody (M377)

SKU: CM3771

Product Details

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